000130502 001__ 130502 000130502 005__ 20240125162931.0 000130502 0247_ $$2doi$$a10.1089/ten.tec.2015.0062 000130502 0248_ $$2sideral$$a92848 000130502 037__ $$aART-2015-92848 000130502 041__ $$aeng 000130502 100__ $$0(orcid)0000-0002-4379-1100$$aLlobet, L.$$uUniversidad de Zaragoza 000130502 245__ $$aSide Effects of Culture Media Antibiotics on Cell Differentiation 000130502 260__ $$c2015 000130502 5203_ $$aBesides the advance in scientific knowledge and the production of different compounds, cell culture can now be used to obtain cells for regenerative medicine. To avoid microbial contamination, antibiotics were usually incorporated into culture media. However, these compounds affect cell biochemistry and may modify the differentiation potential of cultured cells. To check this possibility, we grew human adipose tissue-derived stem cells and differentiated them to adipocyte with or without antibiotics commonly used in these culture protocols, such as a penicillin-streptomycin-amphotericin mix or gentamicin. We show that these antibiotics affect cell differentiation. Therefore, antibiotics should not be used in cell culture because aseptic techniques make these compounds unnecessary. 000130502 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/ 000130502 592__ $$a1.174$$b2015 000130502 593__ $$aBioengineering$$c2015$$dQ1 000130502 593__ $$aMedicine (miscellaneous)$$c2015$$dQ1 000130502 593__ $$aBiomedical Engineering$$c2015$$dQ1 000130502 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000130502 700__ $$0(orcid)0000-0003-1770-6299$$aMontoya, J.$$uUniversidad de Zaragoza 000130502 700__ $$0(orcid)0000-0002-3217-1424$$aLópez-Gallardo, E.$$uUniversidad de Zaragoza 000130502 700__ $$0(orcid)0000-0002-0269-7337$$aRuiz-Pesini, E.$$uUniversidad de Zaragoza 000130502 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología 000130502 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole. 000130502 773__ $$g21, 11 (2015), 1143-1147$$pTissue eng. Part C Methods$$tTissue engineering. Part C Methods$$x1937-3384 000130502 8564_ $$s211936$$uhttps://zaguan.unizar.es/record/130502/files/texto_completo.pdf$$yVersión publicada 000130502 8564_ $$s2621731$$uhttps://zaguan.unizar.es/record/130502/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000130502 909CO $$ooai:zaguan.unizar.es:130502$$particulos$$pdriver 000130502 951__ $$a2024-01-25-15:15:52 000130502 980__ $$aARTICLE