000130502 001__ 130502
000130502 005__ 20240125162931.0
000130502 0247_ $$2doi$$a10.1089/ten.tec.2015.0062
000130502 0248_ $$2sideral$$a92848
000130502 037__ $$aART-2015-92848
000130502 041__ $$aeng
000130502 100__ $$0(orcid)0000-0002-4379-1100$$aLlobet, L.$$uUniversidad de Zaragoza
000130502 245__ $$aSide Effects of Culture Media Antibiotics on Cell Differentiation
000130502 260__ $$c2015
000130502 5203_ $$aBesides the advance in scientific knowledge and the production of different compounds, cell culture can now be used to obtain cells for regenerative medicine. To avoid microbial contamination, antibiotics were usually incorporated into culture media. However, these compounds affect cell biochemistry and may modify the differentiation potential of cultured cells. To check this possibility, we grew human adipose tissue-derived stem cells and differentiated them to adipocyte with or without antibiotics commonly used in these culture protocols, such as a penicillin-streptomycin-amphotericin mix or gentamicin. We show that these antibiotics affect cell differentiation. Therefore, antibiotics should not be used in cell culture because aseptic techniques make these compounds unnecessary.
000130502 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000130502 592__ $$a1.174$$b2015
000130502 593__ $$aBioengineering$$c2015$$dQ1
000130502 593__ $$aMedicine (miscellaneous)$$c2015$$dQ1
000130502 593__ $$aBiomedical Engineering$$c2015$$dQ1
000130502 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000130502 700__ $$0(orcid)0000-0003-1770-6299$$aMontoya, J.$$uUniversidad de Zaragoza
000130502 700__ $$0(orcid)0000-0002-3217-1424$$aLópez-Gallardo, E.$$uUniversidad de Zaragoza
000130502 700__ $$0(orcid)0000-0002-0269-7337$$aRuiz-Pesini, E.$$uUniversidad de Zaragoza
000130502 7102_ $$11003$$2443$$aUniversidad de Zaragoza$$bDpto. Anatom.Histolog.Humanas$$cArea Histología
000130502 7102_ $$11002$$2060$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Bioquímica y Biolog.Mole.
000130502 773__ $$g21, 11 (2015), 1143-1147$$pTissue eng. Part C Methods$$tTissue engineering. Part C Methods$$x1937-3384
000130502 8564_ $$s211936$$uhttps://zaguan.unizar.es/record/130502/files/texto_completo.pdf$$yVersión publicada
000130502 8564_ $$s2621731$$uhttps://zaguan.unizar.es/record/130502/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000130502 909CO $$ooai:zaguan.unizar.es:130502$$particulos$$pdriver
000130502 951__ $$a2024-01-25-15:15:52
000130502 980__ $$aARTICLE