000130738 001__ 130738
000130738 005__ 20240131210810.0
000130738 0247_ $$2doi$$a10.1007/s00216-016-0022-y
000130738 0248_ $$2sideral$$a97006
000130738 037__ $$aART-2017-97006
000130738 041__ $$aeng
000130738 100__ $$0(orcid)0000-0003-4597-4020$$aClemente, Isabel$$uUniversidad de Zaragoza
000130738 245__ $$aRaman spectroscopy, electronic microscopy and SPME-GC-MS to elucidate the mode of action of a new antimicrobial food packaging material
000130738 260__ $$c2017
000130738 5060_ $$aAccess copy available to the general public$$fUnrestricted
000130738 5203_ $$aOne critical challenge when developing a new antimicrobial packaging material is to demonstrate the mode of action of the antimicrobials incorporated into the packaging. For this task, several analytical techniques as well as microbiology are required. In this work, the antimicrobial properties of benzyl isothiocyanate, allyl isothiocyanate and essential oils of cinnamon and oregano against several moulds and bacteria have been evaluated. Benzyl isothiocyanate showed the highest antimicrobial activity and it was selected for developing the new active packaging material. Scanning electron microscopy and Raman spectroscopy were successfully used to demonstrate the mode of action of benzyl isothiocyanate on Escherichia coli. Bacteria exhibited external modifications such as oval shape and the presence of septum surface, but they did not show any disruption or membrane damage. To provide data on the in vitro action of benzyl isothiocyanate and the presence of inhibition halos, the transfer mechanism to the cells was assessed using solid-phase microextraction–gas chromatography–mass spectrometry. Based on the transfer system, action mechanism and its stronger antimicrobial activity, benzyl isothiocyanate was incorporated to two kinds of antimicrobial labels. The labels were stable and active for 140 days against two mould producers of ochratoxin A; Penicillium verrucosum is more sensitive than Aspergillus ochraceus. Details about the analytical techniques and the results obtained are shown and discussed.
000130738 536__ $$9info:eu-repo/grantAgreement/ES/DGA/T10$$9info:eu-repo/grantAgreement/ES/MINECO/RYC-2012-11856$$9info:eu-repo/grantAgreement/ES/UZ/PIFUZ-2012-B-CIE-001
000130738 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000130738 590__ $$a3.307$$b2017
000130738 591__ $$aCHEMISTRY, ANALYTICAL$$b15 / 78 = 0.192$$c2017$$dQ1$$eT1
000130738 591__ $$aBIOCHEMICAL RESEARCH METHODS$$b27 / 77 = 0.351$$c2017$$dQ2$$eT2
000130738 592__ $$a0.978$$b2017
000130738 593__ $$aAnalytical Chemistry$$c2017$$dQ1
000130738 593__ $$aBiochemistry$$c2017$$dQ2
000130738 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000130738 700__ $$0(orcid)0000-0002-0759-9170$$aAznar, Margarita$$uUniversidad de Zaragoza
000130738 700__ $$0(orcid)0000-0002-6607-1309$$aSalafranca, Jesús$$uUniversidad de Zaragoza
000130738 700__ $$0(orcid)0000-0003-2685-5739$$aNerín, Crisitna$$uUniversidad de Zaragoza
000130738 7102_ $$12009$$2750$$aUniversidad de Zaragoza$$bDpto. Química Analítica$$cÁrea Química Analítica
000130738 773__ $$g409, 4 (2017), 1037-1048$$pAnal. Bioanal. Chem.$$tAnalytical and Bioanalytical Chemistry$$x1618-2642
000130738 8564_ $$s751439$$uhttps://zaguan.unizar.es/record/130738/files/texto_completo.pdf$$yPostprint
000130738 8564_ $$s1951935$$uhttps://zaguan.unizar.es/record/130738/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000130738 909CO $$ooai:zaguan.unizar.es:130738$$particulos$$pdriver
000130738 951__ $$a2024-01-31-19:17:13
000130738 980__ $$aARTICLE