Raman spectroscopy, electronic microscopy and SPME-GC-MS to elucidate the mode of action of a new antimicrobial food packaging material

Clemente, Isabel (Universidad de Zaragoza) ; Aznar, Margarita (Universidad de Zaragoza) ; Salafranca, Jesús (Universidad de Zaragoza) ; Nerín, Crisitna (Universidad de Zaragoza)

Resumen: One critical challenge when developing a new antimicrobial packaging material is to demonstrate the mode of action of the antimicrobials incorporated into the packaging. For this task, several analytical techniques as well as microbiology are required. In this work, the antimicrobial properties of benzyl isothiocyanate, allyl isothiocyanate and essential oils of cinnamon and oregano against several moulds and bacteria have been evaluated. Benzyl isothiocyanate showed the highest antimicrobial activity and it was selected for developing the new active packaging material. Scanning electron microscopy and Raman spectroscopy were successfully used to demonstrate the mode of action of benzyl isothiocyanate on Escherichia coli. Bacteria exhibited external modifications such as oval shape and the presence of septum surface, but they did not show any disruption or membrane damage. To provide data on the in vitro action of benzyl isothiocyanate and the presence of inhibition halos, the transfer mechanism to the cells was assessed using solid-phase microextraction–gas chromatography–mass spectrometry. Based on the transfer system, action mechanism and its stronger antimicrobial activity, benzyl isothiocyanate was incorporated to two kinds of antimicrobial labels. The labels were stable and active for 140 days against two mould producers of ochratoxin A; Penicillium verrucosum is more sensitive than Aspergillus ochraceus. Details about the analytical techniques and the results obtained are shown and discussed.

Idioma: Inglés

DOI: 10.1007/s00216-016-0022-y

Año: 2017

Publicado en: Analytical and Bioanalytical Chemistry 409, 4 (2017), 1037-1048
ISSN: 1618-2642

Factor impacto JCR: 3.307 (2017)

Categ. JCR: CHEMISTRY, ANALYTICAL rank: 15 / 78 = 0.192 (2017) - Q1 - T1
Categ. JCR: BIOCHEMICAL RESEARCH METHODS rank: 27 / 77 = 0.351 (2017) - Q2 - T2

Factor impacto SCIMAGO: 0.978 - Analytical Chemistry (Q1) - Biochemistry (Q2)

Financiación: info:eu-repo/grantAgreement/ES/DGA/T10
Financiación: info:eu-repo/grantAgreement/ES/MINECO/RYC-2012-11856
Financiación: info:eu-repo/grantAgreement/ES/UZ/PIFUZ-2012-B-CIE-001

Tipo y forma: Artículo (PostPrint)

Área (Departamento): Área Química Analítica (Dpto. Química Analítica)