Resumen: The use of chilled semen has gained increasing interest in canine reproductive services. The addition of phosphodiesterase (PDE) inhibitors that increase the intracellular cyclic adenosine monophosphate levels may improve sperm motility. The purpose of this study was to examine the quality of sperm under the effect of the specific PDE-10 inhibitor (papaverine) added after storage for 1, 2, and 3 days at 5 °C. The ejaculates were obtained from 5 healthy Beagle dogs by digital manipulation. After collection, ejaculates were pooled, extended and cooled at 5 °C during 3 days. Sperm parameters were tested 30 min after the addition of different papaverine (PA) concentrations: 0, 5, 10 and 20 µM. Sperm motility (CASA), viability (PI/FITC-PNA) and capacitation status (chlortetracycline assay) were evaluated. The results showed that the addition of PA has no effect on sperm samples at day 0. However, concentrations of 5 and 10 µM increased (p < .05) sperm motility kinetics and viability significantly compared to the control at day 1, day 2 and day 3 of cooling. The addition of 20 μM PA decreased (p < .05) sperm quality parameters significantly and increased the percentage of capacitated/acrosome-reacted spermatozoa. In conclusion, the addition of 5 and 10 μM PA concentrations after cooled storage improved canine sperm quality. Idioma: Inglés DOI: 10.1080/10495398.2020.1811714 Año: 2022 Publicado en: ANIMAL BIOTECHNOLOGY 33, 3 (2022), 519-525 ISSN: 1049-5398 Factor impacto JCR: 3.7 (2022) Categ. JCR: AGRICULTURE, DAIRY & ANIMAL SCIENCE rank: 6 / 62 = 0.097 (2022) - Q1 - T1 Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 65 / 158 = 0.411 (2022) - Q2 - T2 Factor impacto CITESCORE: 3.3 - Agricultural and Biological Sciences (Q2) - Chemical Engineering (Q3) - Biochemistry, Genetics and Molecular Biology (Q3)