000130782 001__ 130782
000130782 005__ 20240319080945.0
000130782 0247_ $$2doi$$a10.1080/10495398.2020.1811714
000130782 0248_ $$2sideral$$a119016
000130782 037__ $$aART-2022-119016
000130782 041__ $$aeng
000130782 100__ $$0(orcid)0000-0002-7857-9732$$aLuño, Victoria$$uUniversidad de Zaragoza
000130782 245__ $$aSpecific phosphodiesterase type-10 inhibitor, papaverine, added after the cooling period improves canine sperm quality
000130782 260__ $$c2022
000130782 5060_ $$aAccess copy available to the general public$$fUnrestricted
000130782 5203_ $$aThe use of chilled semen has gained increasing interest in canine reproductive services. The addition of phosphodiesterase (PDE) inhibitors that increase the intracellular cyclic adenosine monophosphate levels may improve sperm motility. The purpose of this study was to examine the quality of sperm under the effect of the specific PDE-10 inhibitor (papaverine) added after storage for 1, 2, and 3 days at 5 °C. The ejaculates were obtained from 5 healthy Beagle dogs by digital manipulation. After collection, ejaculates were pooled, extended and cooled at 5 °C during 3 days. Sperm parameters were tested 30 min after the addition of different papaverine (PA) concentrations: 0, 5, 10 and 20 µM. Sperm motility (CASA), viability (PI/FITC-PNA) and capacitation status (chlortetracycline assay) were evaluated. The results showed that the addition of PA has no effect on sperm samples at day 0. However, concentrations of 5 and 10 µM increased (p < .05) sperm motility kinetics and viability significantly compared to the control at day 1, day 2 and day 3 of cooling. The addition of 20 μM PA decreased (p < .05) sperm quality parameters significantly and increased the percentage of capacitated/acrosome-reacted spermatozoa. In conclusion, the addition of 5 and 10 μM PA concentrations after cooled storage improved canine sperm quality.
000130782 536__ $$9info:eu-repo/grantAgreement/ES/DGA-FEDER/A17-17R-RAySA$$9info:eu-repo/grantAgreement/ES/DGA-FEDER/Construyendo Europa desde Aragón
000130782 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000130782 590__ $$a3.7$$b2022
000130782 591__ $$aAGRICULTURE, DAIRY & ANIMAL SCIENCE$$b6 / 62 = 0.097$$c2022$$dQ1$$eT1
000130782 591__ $$aBIOTECHNOLOGY & APPLIED MICROBIOLOGY$$b65 / 158 = 0.411$$c2022$$dQ2$$eT2
000130782 592__ $$a0.367$$b2022
000130782 593__ $$aAnimal Science and Zoology$$c2022$$dQ2
000130782 593__ $$aBiotechnology$$c2022$$dQ3
000130782 593__ $$aBioengineering$$c2022$$dQ3
000130782 594__ $$a3.3$$b2022
000130782 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000130782 700__ $$0(orcid)0000-0002-1611-6337$$aGonzález, Noelia$$uUniversidad de Zaragoza
000130782 700__ $$0(orcid)0000-0002-1659-3387$$aMartínez, Felisa$$uUniversidad de Zaragoza
000130782 700__ $$aGonzález, Beatriz
000130782 700__ $$0(orcid)0000-0001-6479-3933$$aOlaciregui, Maite
000130782 700__ $$0(orcid)0000-0002-0797-1841$$aGil, Lydia$$uUniversidad de Zaragoza
000130782 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000130782 773__ $$g33, 3 (2022), 519-525$$pAnim. biotechnol.$$tANIMAL BIOTECHNOLOGY$$x1049-5398
000130782 8564_ $$s381016$$uhttps://zaguan.unizar.es/record/130782/files/texto_completo.pdf$$yPostprint
000130782 8564_ $$s556446$$uhttps://zaguan.unizar.es/record/130782/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000130782 909CO $$ooai:zaguan.unizar.es:130782$$particulos$$pdriver
000130782 951__ $$a2024-03-18-12:32:32
000130782 980__ $$aARTICLE