000132065 001__ 132065
000132065 005__ 20240301161205.0
000132065 0247_ $$2doi$$a10.1021/acs.bioconjchem.7b00037
000132065 0248_ $$2sideral$$a98873
000132065 037__ $$aART-2017-98873
000132065 041__ $$aeng
000132065 100__ $$0(orcid)0000-0001-6584-6603$$aLancelot, A.$$uUniversidad de Zaragoza
000132065 245__ $$aDNA transfection to mesenchymal stem cells using a novel type of pseudodendrimer based on 2,2-bis(hydroxymethyl)propionic acid
000132065 260__ $$c2017
000132065 5060_ $$aAccess copy available to the general public$$fUnrestricted
000132065 5203_ $$aIn the search for effective vehicles to carry genetic material into cells, we present here new pseudodendrimers that consist of a hyperbranched polyester core surrounded by amino-terminated 2,2-bis(hydroxymethyl)propionic acid (bis-MPA) dendrons. The pseudodendrimers are readily synthesized from commercial hyperbranched bis-MPA polyesters of the second, third, and fourth generations and third-generation bis-MPA dendrons, bearing eight peripheral glycine moieties, coupled by the copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC). This approach provides globular macromolecular structures bearing 128, 256, and 512 terminal amino groups, and these can complex pDNA. The toxicity of the three pseudodendrimers was studied on two cell lines, mesenchymal stem cells, and HeLa, and it was demonstrated that these compounds do not affect negatively cell viability up to 72 h. The complexation with DNA was investigated in terms of N-to-P ratio and dendriplex stability. The three generations were found to promote internalizing of pDNA into mesenchymal stem cells (MSCs), and their transfection capacity was compared with two nonviral commercial transfection agents, Lipofectamine and TransIT-X2. The highest generations were able to transfect these cells at levels comparable to both commercial reagents.
000132065 536__ $$9info:eu-repo/grantAgreement/ES/DGA/E04$$9info:eu-repo/grantAgreement/ES/MEC/FPU12-05210$$9info:eu-repo/grantAgreement/ES/MINECO/CTQ2015-70174-P$$9info:eu-repo/grantAgreement/ES/MINECO/MAT2015-66208-C3-1-P
000132065 540__ $$9info:eu-repo/semantics/openAccess$$aAll rights reserved$$uhttp://www.europeana.eu/rights/rr-f/
000132065 590__ $$a4.485$$b2017
000132065 591__ $$aBIOCHEMICAL RESEARCH METHODS$$b10 / 77 = 0.13$$c2017$$dQ1$$eT1
000132065 591__ $$aCHEMISTRY, MULTIDISCIPLINARY$$b42 / 169 = 0.249$$c2017$$dQ1$$eT1
000132065 591__ $$aCHEMISTRY, ORGANIC$$b10 / 57 = 0.175$$c2017$$dQ1$$eT1
000132065 591__ $$aBIOCHEMISTRY & MOLECULAR BIOLOGY$$b61 / 292 = 0.209$$c2017$$dQ1$$eT1
000132065 592__ $$a1.801$$b2017
000132065 593__ $$aBioengineering$$c2017$$dQ1
000132065 593__ $$aBiomedical Engineering$$c2017$$dQ1
000132065 593__ $$aPharmacology$$c2017$$dQ1
000132065 593__ $$aOrganic Chemistry$$c2017$$dQ1
000132065 593__ $$aPharmaceutical Science$$c2017$$dQ1
000132065 593__ $$aBiotechnology$$c2017$$dQ1
000132065 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000132065 700__ $$0(orcid)0000-0002-5630-6645$$aGonzález-Pastor, R.$$uUniversidad de Zaragoza
000132065 700__ $$0(orcid)0000-0002-8932-9085$$aConcellón, A.$$uUniversidad de Zaragoza
000132065 700__ $$0(orcid)0000-0001-7091-077X$$aSierra, T.$$uUniversidad de Zaragoza
000132065 700__ $$0(orcid)0000-0003-4160-9720$$aMartín-Duque, P.
000132065 700__ $$0(orcid)0000-0001-9866-6633$$aSerrano, J. L.$$uUniversidad de Zaragoza
000132065 7102_ $$12013$$2765$$aUniversidad de Zaragoza$$bDpto. Química Orgánica$$cÁrea Química Orgánica
000132065 773__ $$g28, 4 (2017), 1135-1150$$pBioconjug. chem.$$tBioconjugate Chemistry$$x1043-1802
000132065 8564_ $$s2403325$$uhttps://zaguan.unizar.es/record/132065/files/texto_completo.pdf$$yPostprint
000132065 8564_ $$s922608$$uhttps://zaguan.unizar.es/record/132065/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000132065 909CO $$ooai:zaguan.unizar.es:132065$$particulos$$pdriver
000132065 951__ $$a2024-03-01-14:36:38
000132065 980__ $$aARTICLE