Assessing the influence of small structural modifications in simple DNA-based nanostructures on their role as drug nanocarriers

Postigo, Alejandro ; Martínez-Vicente, Pablo (Universidad de Zaragoza) ; Baumann, Kevin N. ; Barrio, Jesús del (Universidad de Zaragoza) ; Hernández-Ainsa, Silvia

Resumen: DNA nanotechnology leverages Watson–Crick–Franklin base-pairing interactions to build complex DNA-based nanostructures (DNS). Due to DNA specific self-assembly properties, DNS can be designed with a total control of their architecture, which has been demonstrated to have an impact on the overall DNS features. Indeed, structural properties such as the shape, size and flexibility of DNS can influence their biostability as well as their ability to internalise into cells. We present here two series of simple DNS with small and precise variations related to their length or flexibility and study the influence that these structural changes have on their overall properties as drug nanocarriers. Results indicate that shorter and more flexible DNS present higher stability towards nuclease degradation. These structural changes also have a certain effect on their cell internalisation ability and drug release rate. Consequently, drug-loaded DNS cytotoxicity varies according to the design, with lower cell viability values obtained in the DNS exhibiting faster drug release and larger cell interaction rates. In summary, small changes in the structure of simple DNS can have an influence on their overall capabilities as drug nanocarriers. The effects reported here could guide the design of simple DNS for future therapeutic uses.

Idioma: Inglés

DOI: 10.1039/d3bm01987j

Año: 2024

Publicado en: Biomaterials Science 12, 6 (2024), 1549-1557
ISSN: 2047-4830

Financiación: info:eu-repo/grantAgreement/ES/DGA/E47-23R
Financiación: info:eu-repo/grantAgreement/ES/DGA-FSE/E47-20R
Financiación: info:eu-repo/grantAgreement/ES/DGA/LMP128_21
Financiación: info:eu-repo/grantAgreement/EC/H2020/853468/EU/Remote control of cellular signalling triggered by magnetic switching/SIROCCO
Financiación: info:eu-repo/grantAgreement/ES/MICINN/CTQ2017-84087-R
Financiación: info:eu-repo/grantAgreement/ES/MCINN/PID2020-113003GB-I00
Financiación: info:eu-repo/grantAgreement/ES/MICINN/RYC-2015-18471

Tipo y forma: Article (Published version)

Área (Departamento): Área Química Orgánica (Dpto. Química Orgánica)
Área (Departamento): Área Toxicología (Dpto. Bioq.Biolog.Mol. Celular)