Effect of the dietary administration pattern of silver nanoparticles on growth performance, biodiversity of digestive microbiota and tissue retention in broiler chickens

Zaoui, Yahya ; Belanche, Alejandro (Universidad de Zaragoza) ; Ben-Jeddou, Khaoula ; Jiménez García-Alcalá, María Sierra (Universidad de Zaragoza) ; Fondevila, Guillermo (Universidad de Zaragoza) ; Fondevila, Manuel (Universidad de Zaragoza)

Resumen: The effects of dietary inclusion of silver nanoparticles (NanoAg, <100 nm diameter) on growth performance, gut microbiota, and silver tissue retention was assessed in broilers from 1 to 42 days of age. A total of 870 1-day-old male broilers (Ross 308) were weighted and distributed in 36 floor pens (n = 24) in an environmentally controlled room. The feeding program consisted of two periods (1–21 days and 22 to 42 days of age) and two experimental diets with the same ingredient composition including 2 g kaolin/kg with or without adsorbed NanoAg (10 mg/g kaolin). The experimental design was completely randomized, and the effects of the length of the period in which the birds received NanoAg (none, Ag0; from 1 to 21 days, Ag21; or from 1 to 35 days, Ag35) on growth performance, biodiversity of digestive microbiota and silver retention in body tissues were studied. A common feed without NanoAg was provided to all pens from 36 days onwards. At 21 and 42 days of, one random bird per pen (n = 12) was slaughtered and cecal samples were collected from 9 birds per treatment randomly selected to analyse gut microbiota. Besides, samples of liver and breast muscle were collected to determine silver tissue retention. From 1 to 21 days of age, NanoAg supplementation tended to improve feed conversion ratio (FCR, P = 0.070). From 22 to 35 days of age, FCR tended to be lower (P = 0.072) and average daily gain (ADG) was greater (P < 0.001) in broilers fed Ag21 and Ag35 than in those fed Ag0. Cumulatively, ADG was greater (P < 0.001) for Ag21 and Ag35 than for Ag0, but FCR was unaffected. Caecal microbiota was affected by age of birds, but dietary supplementation with NanoAg did not modify bacterial community structure, diversity and taxa distribution in the caecum neither at 21 nor at 42 days. At 21 days of age, silver retention in liver was 0.591 mg/kg dry tissue in all broilers supplemented with NanoAg. However, at 42 days of age, silver retention in the liver was only detected in two (0.139 mg/kg, n = 2) and 8 (0.183 mg/kg, n = 8) birds fed Ag21 and Ag35, respectively. Silver retention in muscle was exclusively detected at 42 days in two birds (0.124 mg/kg, n = 2) fed Ag35. Irrespective of the supplementation period, NanoAg increased ADG from 1 to 42 d. No silver retention was detected in the liver nor in breast muscle after 21 days of the end of treatment. However, traces of silver might remain in the liver 7 days after removal of NanoAg. Silver supplementation might be a promising strategy to improve growth performance in broilers without expecting any changes in the gut microbiota nor tissue retention after 21 days of NanoAg withdrawal.

Idioma: Inglés

DOI: 10.1016/j.anifeedsci.2024.115888

Año: 2024

Publicado en: Animal Feed Science and Technology 309 (2024), 115888 [9 pp.]
ISSN: 0377-8401

Factor impacto JCR: 2.7 (2024)

Categ. JCR: AGRICULTURE, DAIRY & ANIMAL SCIENCE rank: 15 / 86 = 0.174 (2024) - Q1 - T1

Factor impacto SCIMAGO: 0.694 - Animal Science and Zoology (Q1)

Financiación: info:eu-repo/grantAgreement/EC/H2020/801586/EU/International Doctoral Programme for Talent Attraction to the Campus of International Excellence of the Ebro Valley/IberusTalent
Financiación: info:eu-repo/grantAgreement/EUR/INTERREG POCTEFA-EFA 183-16
Financiación: info:eu-repo/grantAgreement/ES/MICINN/RYC2019-027764-I/AEI/10.13039/501100011033

Tipo y forma: Article (Published version)

Área (Departamento): Área Química Analítica (Dpto. Química Analítica)
Área (Departamento): Área Producción Animal (Dpto. Produc.Animal Cienc.Ali.)