Sex differences on constitutive long non-coding RNA expression: Modulatory effect of estradiol and testosterone in muscle cells

López-Royo, Tresa (Universidad de Zaragoza) ; Moreno-Martínez, Laura (Universidad de Zaragoza) ; Moreno-García, Leticia ; Calvo, Ana Cristina (Universidad de Zaragoza) ; Manzano, Raquel (Universidad de Zaragoza) ; Osta, Rosario (Universidad de Zaragoza)

Resumen: AbstractIntroductionDespite the growing awareness of sexual dimorphism between males and females under pathological and physiological conditions, sex bias in biomedical research in animal models and patients is still present nowadays. The main objective of this work was to investigate sex differences in constitutive long non‐coding RNA expression in spinal cord and skeletal muscle from wild‐type mice.Materials and MethodsTo assess the influence of gender on long non‐coding RNAs, we extracted RNA from tissues of male and female mice and analyzed the expression on nine long non‐coding RNAs, selected for being among the most commonly studied or exerting an important role in muscle, at 50, 60, and 120 days of age.Results and DiscussionWe observed age‐ and tissue‐dependent significant sex differences, being more prominent in skeletal muscle. We also studied the effect of sex steroid hormones on long non‐coding RNA expression in vitro, noticing a modulation of long non‐coding RNA levels upon estradiol and dihydrotestosterone treatment in muscle.ConclusionsTaken together, results obtained evidenced sex differences on constitutive long non‐coding RNA expression and suggested an influence of steroid hormones complementary to other possible factors. These findings emphasize the importance of including both sexes in experimental design to minimize any potential sex bias.

Idioma: Inglés

DOI: 10.1111/andr.13624

Año: 2024

Publicado en: Andrology 12, 8 (2024), 1887-1896
ISSN: 2047-2919

Financiación: info:eu-repo/grantAgreement/ES/ISCIII/FEDER/PI21-00372

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Genética (Dpto. Anatom.,Embri.Genét.Ani.)
Área (Departamento): Área Farmacología (Dpto. Farmac.Fisiol.y Med.L.F.)