A microphysiological system for handling graphene related materials under flow conditions

Lacueva-Aparicio, Alodia ; González, Viviana Jehová ; Remacha, Ana Rosa ; Woods, Daniel ; Prado, Eduardo ; Ochoa, Ignacio (Universidad de Zaragoza) ; Oliván, Sara (Universidad de Zaragoza) ; Vázquez, Ester

Resumen: The field of nanotechnology has developed rapidly in recent decades due to its broad applications in many industrial and biomedical fields. Notably, 2D materials such as graphene-related materials (GRMs) have been extensively explored and, as such, their safety needs to be assessed. However, GRMs tend to deposit quickly, present low stability in aqueous solutions, and adsorb to plastic materials. Consequently, traditional approaches based on static assays facilitate their deposition and adsorption and fail to recreate human physiological conditions. Organ-on-a-chip (OOC) technology could, however, solve these drawbacks and lead to the development of microphysiological systems (MPSs) that mimic the microenvironment present in human tissues. In light of the above, in the present study a microfluidic system under flow conditions has been optimised to minimise graphene oxide (GO) and few-layer graphene (FLG) adsorption and deposition. For that purpose, a kidney-on-a-chip was developed and optimised to evaluate the effects of exposure to GO and FLG flakes at a sublethal dose under fluid flow conditions. In summary, MPSs are an innovative and precise tool for evaluating the effects of exposure to GRMs and other type of nanomaterials.

Idioma: Inglés

DOI: 10.1039/d4nh00064a

Año: 2024

Publicado en: Nanoscale Horizons 9, 6 (2024), 990-1001
ISSN: 2055-6756

Financiación: info:eu-repo/grantAgreement/EC/H2020/881603/EU/Graphene Flagship Core Project 3/GrapheneCore3
Financiación: info:eu-repo/grantAgreement/ES/MICINN/AEI/PID2020-113080RB-I00

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Area Histología (Dpto. Anatom.Histolog.Humanas)