Serum protein electrophoresis in European mink (Mustela lutreola): reference intervals and comparison of agarose gel electrophoresis and capillary zone electrophoresis
Villanueva-Saz, Sergio (Universidad de Zaragoza) ; Aranda, María del Carmen ; Jiménez, María de los Ángeles ; de Andrés, Paloma Jimena ; Verde, Maite (Universidad de Zaragoza) ; Climent, María (Universidad de Zaragoza) ; Lebrero Berna, María Eugenia (Universidad de Zaragoza) ; Marteles Aragüés, Diana (Universidad de Zaragoza) ; Fernández, Antonio (Universidad de Zaragoza)
Resumen: Background
Knowledge of reference intervals for blood analytes, including serum protein fractions, is of great importance for the identification of infectious and inflammatory diseases and is often lacking in wild animal species.
Material and methods
Serum samples were obtained from European minks enrolled in the breeding program (n = 55). Agarose gel electrophoresis (AGE) and capillary zone electrophoresis (CZE) were used to separate and identify protein fractions. Albumin, α1, α2, β, and γ-globulins fractions were identified in all mink sera by both electrophoresis methods. Reference intervals (90% CI) were determined following the 2008 guidelines of the Clinical Laboratory Standard Institute. The methods were compared using Passing-Bablok regression, Bland–Altman analysis, and Lin’s concordance correlation.
Results
A significant bias was found between methods for α1, α2, and γ-globulin. Lin’s concordance correlation was considered unacceptable for α1, α2, and β-globulins. Differences for gender between methods were found for albumin and α2-globuins, which were higher for males than females. γ-globulins were higher for adults than young minks using both methods; however, α1 and α2-globulins were lower.
Conclusion
Both methods are adequate for identifying serum protein disorders, but the AGE and CZE methods are not equivalent. Therefore, reference intervals for each technique are required.
Idioma: Inglés
DOI: 10.1080/01652176.2024.2318195
Año: 2024
Publicado en: VETERINARY QUARTERLY 44, 1 (2024), [11 pp.]
ISSN: 0165-2176
Tipo y forma: Article (Published version)
Área (Departamento): Área Anatom.Anatom.Patológ.Com (Dpto. Anatom.,Embri.Genét.Ani.)
Área (Departamento): Área Medicina y Cirugía Animal (Dpto. Patología Animal)
Exportado de SIDERAL (2024-05-03-11:07:01)
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Knowledge of reference intervals for blood analytes, including serum protein fractions, is of great importance for the identification of infectious and inflammatory diseases and is often lacking in wild animal species.
Material and methods
Serum samples were obtained from European minks enrolled in the breeding program (n = 55). Agarose gel electrophoresis (AGE) and capillary zone electrophoresis (CZE) were used to separate and identify protein fractions. Albumin, α1, α2, β, and γ-globulins fractions were identified in all mink sera by both electrophoresis methods. Reference intervals (90% CI) were determined following the 2008 guidelines of the Clinical Laboratory Standard Institute. The methods were compared using Passing-Bablok regression, Bland–Altman analysis, and Lin’s concordance correlation.
Results
A significant bias was found between methods for α1, α2, and γ-globulin. Lin’s concordance correlation was considered unacceptable for α1, α2, and β-globulins. Differences for gender between methods were found for albumin and α2-globuins, which were higher for males than females. γ-globulins were higher for adults than young minks using both methods; however, α1 and α2-globulins were lower.
Conclusion
Both methods are adequate for identifying serum protein disorders, but the AGE and CZE methods are not equivalent. Therefore, reference intervals for each technique are required.
Idioma: Inglés
DOI: 10.1080/01652176.2024.2318195
Año: 2024
Publicado en: VETERINARY QUARTERLY 44, 1 (2024), [11 pp.]
ISSN: 0165-2176
Tipo y forma: Article (Published version)
Área (Departamento): Área Anatom.Anatom.Patológ.Com (Dpto. Anatom.,Embri.Genét.Ani.)
Área (Departamento): Área Medicina y Cirugía Animal (Dpto. Patología Animal)
Exportado de SIDERAL (2024-05-03-11:07:01)
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Notice créée le 2024-05-03, modifiée le 2024-05-03