Evaluation of a Method for Rapid Detection of Listeria monocytogenes in Dry-Cured Ham Based on Impedanciometry Combined with Chromogenic Agar

Labrador, M. (Universidad de Zaragoza) ; Rota, M.C. (Universidad de Zaragoza) ; Perez, C. (Universidad de Zaragoza) ; Herrera, A. (Universidad de Zaragoza) ; Bayarri, S. (Universidad de Zaragoza)

Resumen: The food industry is in need of rapid, reliable methodologies for the detection of Listeria monocytogenes in ready-to-eat products, as an alternative to the International Organization of Standardization (ISO) 11290-1 reference method. The aim of this study was to evaluate impedanciometry combined with chromogenic agar culture for the detection of L. monocytogenes in dry-cured ham. The experimental setup consisted in assaying four strains of L. monocytogenes and two strains of Listeria innocua in pure culture. The method was evaluated according to the ISO 16140:2003 standard through a comparative study with the ISO reference method with 119 samples of dry-cured ham. Significant determination coefficients (R-2 of up to 0.99) for all strains assayed in pure culture were obtained. The comparative study results had 100% accuracy, 100% specificity, and 100% sensitivity. Impedanciometry followed by chromogenic agar culture was capable of detecting 1 CFU/25 g of food. L. monocytogenes was not detected in the 65 commercial samples tested. The method evaluated herein represents a promising alternative for the food industry in its efforts to control L. monocytogenes. Overall analysis time is shorter and the method permits a straightforward analysis of a large number of samples with reliable results.

Idioma: Inglés

DOI: 10.4315/0362-028X.JFP-17-380

Año: 2018

Publicado en: JOURNAL OF FOOD PROTECTION 81, 5 (2018), 705-712
ISSN: 0362-028X

Factor impacto JCR: 1.559 (2018)

Categ. JCR: FOOD SCIENCE & TECHNOLOGY rank: 76 / 135 = 0.563 (2018) - Q3 - T2
Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 125 / 162 = 0.772 (2018) - Q4 - T3

Factor impacto SCIMAGO: 0.613 - Microbiology (Q2) - Food Science (Q2)

Financiación: info:eu-repo/grantAgreement/ES/MINECO/INNPACTO-IPT-2012-0189-060000

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Nutrición Bromatología (Dpto. Produc.Animal Cienc.Ali.)