000135590 001__ 135590
000135590 005__ 20240605121015.0
000135590 0247_ $$2doi$$a10.1364/BOE.509227
000135590 0248_ $$2sideral$$a138702
000135590 037__ $$aART-2024-138702
000135590 041__ $$aeng
000135590 100__ $$aBueno, Juan M.
000135590 245__ $$aAnalysis of age-related changes in the left ventricular myocardium with multiphoton microscopy
000135590 260__ $$c2024
000135590 5060_ $$aAccess copy available to the general public$$fUnrestricted
000135590 5203_ $$aAging induces cardiac remodeling, resulting in an increase in the risk of suffering heart diseases, including heart failure. Collagen deposition increases with age and, together with sarcomeric changes in cardiomyocytes, may lead to ventricular stiffness. Multiphoton (MP) microscopy is a useful technique to visualize and detect variations in cardiac structures in a label free fashion. Here, we propose a method based on MP imaging (both two-photon excitation fluorescence (TPEF) and second harmonic generation (SHG) modalities) to explore and objectively quantify age-related structural differences in various components of cardiac tissues. Results in transmural porcine left ventricle (LV) sections reveal significant differences when comparing samples from young and old animals. Collagen and myosin SHG signals in old specimens are respectively 3.8x and >6-fold larger than in young ones. Differences in TPEF signals from cardiomyocyte were ∼3x. Moreover, the increased amount of collagen in old specimens results in a more organized pattern when compared to young LV tissues. Since changes in collagen and myosin are associated with cardiac dysfunction, the technique used herein might be a useful tool to accurately predict and measure changes associated with age-related myocardium fibrosis, tissue remodeling and sarcomeric alterations, with potential implications in preventing heart disease.
000135590 536__ $$9info:eu-repo/grantAgreement/ES/AEI/PID2020-113919RB-I00$$9info:eu-repo/grantAgreement/ES/MCINN/PID2022-139859OB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2022-1405560B-I00$$9info:eu-repo/grantAgreement/EUR/MICINN/TED2021-130459B-I00$$9info:eu-repo/grantAgreement/ES/MINECO/PID2019-105674RB-I00
000135590 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000135590 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000135590 700__ $$aMartínez-Ojeda, Rosa M.
000135590 700__ $$0(orcid)0000-0002-3194-7796$$aPérez-Zabalza, María
000135590 700__ $$0(orcid)0000-0002-2954-1068$$aGarcía-Mendívil, Laura
000135590 700__ $$aAsensio, M. Carmen
000135590 700__ $$0(orcid)0000-0003-3982-1263$$aOrdovás, Laura
000135590 700__ $$0(orcid)0000-0002-1960-407X$$aPueyo, Esther$$uUniversidad de Zaragoza
000135590 7102_ $$15008$$2800$$aUniversidad de Zaragoza$$bDpto. Ingeniería Electrón.Com.$$cÁrea Teoría Señal y Comunicac.
000135590 773__ $$g15, 5 (2024), 3251-3264$$pBIOMEDICAL OPTICS EXPRESS$$tBiomedical Optics Express$$x2156-7085
000135590 8564_ $$s6972192$$uhttps://zaguan.unizar.es/record/135590/files/texto_completo.pdf$$yVersión publicada
000135590 8564_ $$s2382051$$uhttps://zaguan.unizar.es/record/135590/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000135590 909CO $$ooai:zaguan.unizar.es:135590$$particulos$$pdriver
000135590 951__ $$a2024-06-05-10:51:06
000135590 980__ $$aARTICLE