Biofilm on total joint replacement materials can be reduced through electromagnetic induction heating using a portable device
Enrique, Cordero García-Galán ; Medel-Plaza, Marina ; Correa, John Jairo Aguilera ; Sarnago, Héctor (Universidad de Zaragoza) ; Acero, Jesús (Universidad de Zaragoza) ; Burdio, José M. (Universidad de Zaragoza) ; Lucía, Óscar (Universidad de Zaragoza) ; Esteban, Jaime ; Gómez-Barrena, Enrique
Resumen: Background
periprosthetic joint infection is a serious complication following joint replacement. The development of bacterial biofilms bestows antibiotic resistance and restricts treatment via implant retention surgery. Electromagnetic induction heating is a novel technique for antibacterial treatment of metallic surfaces that has demonstrated in-vitro efficacy. Previous studies have always employed stationary, non-portable devices. This study aims to assess the in-vitro efficacy of induction-heating disinfection of metallic surfaces using a new Portable Disinfection System based on Induction Heating.
Methods
Mature biofilms of three bacterial species: S. epidermidis ATCC 35,984, S. aureus ATCC 25,923, E. coli ATCC 25,922, were grown on 18 × 2 mm cylindrical coupons of Titanium-Aluminium-Vanadium (Ti6Al4V) or Cobalt-chromium-molybdenum (CoCrMo) alloys. Study intervention was induction-heating of the coupon surface up to 70ºC for 210s, performed using the Portable Disinfection System (PDSIH). Temperature was monitored using thermographic imaging. For each bacterial strain and each metallic alloy, experiments and controls were conducted in triplicate. Bacterial load was quantified through scraping and drop plate techniques. Data were evaluated using non-parametric Mann-Whitney U test for 2 group comparison. Statistical significance was fixed at p ≤ 0.05.
Results
All bacterial strains showed a statistically significant reduction of CFU per surface area in both materials. Bacterial load reduction amounted to 0.507 and 0.602 Log10 CFU/mL for S. aureus on Ti6Al4V and CoCrMo respectively, 5.937 and 3.500 Log10 CFU/mL for E. coli, and 1.222 and 0.372 Log10 CFU/mL for S. epidermidis.
Conclusions
Electromagnetic induction heating using PDSIH is efficacious to reduce mature biofilms of S aureus, E coli and S epidermidis growing on metallic surfaces of Ti6Al4V and CoCrMo alloys.
Idioma: Inglés
DOI: 10.1186/s13018-024-04785-x
Año: 2024
Publicado en: Journal of Orthopaedic Surgery and Research 19, 1 (2024), 304 [7 pp.]
ISSN: 1749-799X
Tipo y forma: Article (Published version)
Área (Departamento): Área Tecnología Electrónica (Dpto. Ingeniería Electrón.Com.)
Exportado de SIDERAL (2024-06-14-09:00:02)
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periprosthetic joint infection is a serious complication following joint replacement. The development of bacterial biofilms bestows antibiotic resistance and restricts treatment via implant retention surgery. Electromagnetic induction heating is a novel technique for antibacterial treatment of metallic surfaces that has demonstrated in-vitro efficacy. Previous studies have always employed stationary, non-portable devices. This study aims to assess the in-vitro efficacy of induction-heating disinfection of metallic surfaces using a new Portable Disinfection System based on Induction Heating.
Methods
Mature biofilms of three bacterial species: S. epidermidis ATCC 35,984, S. aureus ATCC 25,923, E. coli ATCC 25,922, were grown on 18 × 2 mm cylindrical coupons of Titanium-Aluminium-Vanadium (Ti6Al4V) or Cobalt-chromium-molybdenum (CoCrMo) alloys. Study intervention was induction-heating of the coupon surface up to 70ºC for 210s, performed using the Portable Disinfection System (PDSIH). Temperature was monitored using thermographic imaging. For each bacterial strain and each metallic alloy, experiments and controls were conducted in triplicate. Bacterial load was quantified through scraping and drop plate techniques. Data were evaluated using non-parametric Mann-Whitney U test for 2 group comparison. Statistical significance was fixed at p ≤ 0.05.
Results
All bacterial strains showed a statistically significant reduction of CFU per surface area in both materials. Bacterial load reduction amounted to 0.507 and 0.602 Log10 CFU/mL for S. aureus on Ti6Al4V and CoCrMo respectively, 5.937 and 3.500 Log10 CFU/mL for E. coli, and 1.222 and 0.372 Log10 CFU/mL for S. epidermidis.
Conclusions
Electromagnetic induction heating using PDSIH is efficacious to reduce mature biofilms of S aureus, E coli and S epidermidis growing on metallic surfaces of Ti6Al4V and CoCrMo alloys.
Idioma: Inglés
DOI: 10.1186/s13018-024-04785-x
Año: 2024
Publicado en: Journal of Orthopaedic Surgery and Research 19, 1 (2024), 304 [7 pp.]
ISSN: 1749-799X
Tipo y forma: Article (Published version)
Área (Departamento): Área Tecnología Electrónica (Dpto. Ingeniería Electrón.Com.)
Exportado de SIDERAL (2024-06-14-09:00:02)
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Este artículo se encuentra en las siguientes colecciones:
articulos > articulos-por-area > tecnologia_electronica
Notice créée le 2024-06-14, modifiée le 2024-06-14