Effect of ultra-high pressure homogenization on the antirotaviral activity of bovine milk whey

Graikini, Dimitra (Universidad de Zaragoza) ; Alvarez-Sabatel, Saioa ; Puértolas, Eduardo ; Pérez, María Dolores (Universidad de Zaragoza) ; Sánchez, Lourdes (Universidad de Zaragoza)

Resumen: In the present study, ultra-high pressure homogenization (UHPH, 100–250 MPa) was applied on bovine whey. The ability of UHPH-treated whey to neutralize the bovine rotavirus strain WC3 was evaluated in vitro using a model of human intestinal epithelium. Results showed that whey homogenized at 100 and 200 MPa inhibited the rotavirus infection in a dose-response way, exhibiting >95% neutralization at 1.5 mg mL−1 protein concentration. However, after homogenization of whey at 250 MPa, a clear neutralization pattern could not be observed. This could be attributed to aggregation and/or denaturation of some bioactive proteins, as it has been evidenced in the electrophoretic profile of that sample. Interestingly, the fat fraction obtained from all UHPH-treated wheys, showed almost complete neutralization at 1 mg mL−1 protein concentration. Therefore, whey fractions could be used as functional ingredients in novel non-thermal processed products for the control of viral infections.
Industrial relevance
This study provides useful information to increase the commercial value of sweet, skimmed whey as a functional ingredient and its recognition as a natural source of antivirals. The use of emerging processing technologies, such as UHPH, could favor the preservation of certain bioactivity within the dairy by-product.

Idioma: Inglés

DOI: 10.1016/j.ifset.2024.103745

Año: 2024

Publicado en: INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES 96 (2024), 103745 [pp.]
ISSN: 1466-8564

Factor impacto JCR: 6.8 (2024)

Categ. JCR: FOOD SCIENCE & TECHNOLOGY rank: 20 / 181 = 0.11 (2024) - Q1 - T1

Factor impacto SCIMAGO: 1.502 - Chemistry (miscellaneous) (Q1) - Industrial and Manufacturing Engineering (Q1) - Food Science (Q1)

Financiación: info:eu-repo/grantAgreement/ES/DGA/A20-23R
Financiación: info:eu-repo/grantAgreement/EC/H2020/801586/EU/International Doctoral Programme for Talent Attraction to the Campus of International Excellence of the Ebro Valley/IberusTalent
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2022-139104OB-I00
Financiación: info:eu-repo/grantAgreement/ES/MINECO/AGL2017-82987-R

Tipo y forma: Article (Published version)

Área (Departamento): Área Tecnología de Alimentos (Dpto. Produc.Animal Cienc.Ali.)