Differential effect of melatonin on ram spermatozoa depending on the allelic variant of the RsaI polymorphism of the MTR1A gene, incubation medium and season
Peña-Delgado, Victoria (Universidad de Zaragoza) ; Noya, Agustí (Universidad de Zaragoza) ; Carvajal-Serna, Melissa (Universidad de Zaragoza) ; Abecia, José A. (Universidad de Zaragoza) ; Pérez-Pe, Rosaura (Universidad de Zaragoza) ; Casao, Adriana (Universidad de Zaragoza)
Resumen: Context
The RsaI polymorphism of the melatonin receptor MTNR1A gene affects seasonal reproduction in sheep, but its effect on ram spermatozoa and their response to melatonin is unknown.
Aims
This study aims to evaluate whether RsaI polymorphism of the MTNR1A gene influences the response of ram spermatozoa to in vitro added melatonin.
Methods
Spermatozoa from rams carrying different RsaI allelic variants were incubated with melatonin in a TALP medium or a capacitation-triggering medium during the reproductive and non-reproductive seasons. After incubation, sperm motility, membrane integrity, mitochondria activity, oxidative damage, apoptotic markers and capacitation status were assessed.
Key results
In the reproductive season, the T/T genotype was related to some adverse effects of melatonin when spermatozoa were incubated in TALP medium, whereas the C/C genotype was linked with adverse effects when the hormone was added in a capacitation-triggering medium. The decapacitating effect of melatonin on spermatozoa was also different depending on genotype.
Conclusions
The melatonin effect on spermatozoa from rams carrying different RsaI genotypes differed depending on the season and the medium.
Implications
The knowledge of the RsaI allelic variant of the MTNR1A gene of rams could be helpful when carrying out in vitro reproductive techniques in the ovine species.
Idioma: Inglés
DOI: 10.1071/RD23233
Año: 2024
Publicado en: REPRODUCTION FERTILITY AND DEVELOPMENT 36, 10 (2024), [12 pp.]
ISSN: 1031-3613
Financiación: info:eu-repo/grantAgreement/ES/DGA/A07-23R
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2020-113239RB-I00
Tipo y forma: Artículo (Versión definitiva)
Área (Departamento): Área Zoología (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Área Producción Animal (Dpto. Produc.Animal Cienc.Ali.)
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The RsaI polymorphism of the melatonin receptor MTNR1A gene affects seasonal reproduction in sheep, but its effect on ram spermatozoa and their response to melatonin is unknown.
Aims
This study aims to evaluate whether RsaI polymorphism of the MTNR1A gene influences the response of ram spermatozoa to in vitro added melatonin.
Methods
Spermatozoa from rams carrying different RsaI allelic variants were incubated with melatonin in a TALP medium or a capacitation-triggering medium during the reproductive and non-reproductive seasons. After incubation, sperm motility, membrane integrity, mitochondria activity, oxidative damage, apoptotic markers and capacitation status were assessed.
Key results
In the reproductive season, the T/T genotype was related to some adverse effects of melatonin when spermatozoa were incubated in TALP medium, whereas the C/C genotype was linked with adverse effects when the hormone was added in a capacitation-triggering medium. The decapacitating effect of melatonin on spermatozoa was also different depending on genotype.
Conclusions
The melatonin effect on spermatozoa from rams carrying different RsaI genotypes differed depending on the season and the medium.
Implications
The knowledge of the RsaI allelic variant of the MTNR1A gene of rams could be helpful when carrying out in vitro reproductive techniques in the ovine species.
Idioma: Inglés
DOI: 10.1071/RD23233
Año: 2024
Publicado en: REPRODUCTION FERTILITY AND DEVELOPMENT 36, 10 (2024), [12 pp.]
ISSN: 1031-3613
Financiación: info:eu-repo/grantAgreement/ES/DGA/A07-23R
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2020-113239RB-I00
Tipo y forma: Artículo (Versión definitiva)
Área (Departamento): Área Zoología (Dpto. Bioq.Biolog.Mol. Celular)
Área (Departamento): Área Producción Animal (Dpto. Produc.Animal Cienc.Ali.)
Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace. No puede utilizar el material para una finalidad comercial. Si remezcla, transforma o crea a partir del material, no puede difundir el material modificado.Exportado de SIDERAL (2024-08-29-10:45:20)
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Registro creado el 2024-08-29, última modificación el 2024-08-29