000144590 001__ 144590
000144590 005__ 20240829125213.0
000144590 0247_ $$2doi$$a10.1071/RD23233
000144590 0248_ $$2sideral$$a139389
000144590 037__ $$aART-2024-139389
000144590 041__ $$aeng
000144590 100__ $$0(orcid)0000-0002-4670-3735$$aPeña-Delgado, Victoria$$uUniversidad de Zaragoza
000144590 245__ $$aDifferential effect of melatonin on ram spermatozoa depending on the allelic variant of the RsaI polymorphism of the MTR1A gene, incubation medium and season
000144590 260__ $$c2024
000144590 5060_ $$aAccess copy available to the general public$$fUnrestricted
000144590 5203_ $$aContext
The RsaI polymorphism of the melatonin receptor MTNR1A gene affects seasonal reproduction in sheep, but its effect on ram spermatozoa and their response to melatonin is unknown.
Aims
This study aims to evaluate whether RsaI polymorphism of the MTNR1A gene influences the response of ram spermatozoa to in vitro added melatonin.
Methods
Spermatozoa from rams carrying different RsaI allelic variants were incubated with melatonin in a TALP medium or a capacitation-triggering medium during the reproductive and non-reproductive seasons. After incubation, sperm motility, membrane integrity, mitochondria activity, oxidative damage, apoptotic markers and capacitation status were assessed.
Key results
In the reproductive season, the T/T genotype was related to some adverse effects of melatonin when spermatozoa were incubated in TALP medium, whereas the C/C genotype was linked with adverse effects when the hormone was added in a capacitation-triggering medium. The decapacitating effect of melatonin on spermatozoa was also different depending on genotype.
Conclusions
The melatonin effect on spermatozoa from rams carrying different RsaI genotypes differed depending on the season and the medium.
Implications
The knowledge of the RsaI allelic variant of the MTNR1A gene of rams could be helpful when carrying out in vitro reproductive techniques in the ovine species.
000144590 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A07-23R$$9info:eu-repo/grantAgreement/ES/MICINN/PID2020-113239RB-I00
000144590 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/
000144590 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000144590 700__ $$0(orcid)0000-0002-9552-4709$$aNoya, Agustí$$uUniversidad de Zaragoza
000144590 700__ $$0(orcid)0000-0003-3929-5064$$aCarvajal-Serna, Melissa$$uUniversidad de Zaragoza
000144590 700__ $$0(orcid)0000-0003-2827-3054$$aAbecia, José A.$$uUniversidad de Zaragoza
000144590 700__ $$0(orcid)0000-0002-2312-6402$$aPérez-Pe, Rosaura$$uUniversidad de Zaragoza
000144590 700__ $$0(orcid)0000-0003-1997-4262$$aCasao, Adriana$$uUniversidad de Zaragoza
000144590 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología
000144590 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal
000144590 773__ $$g36, 10 (2024), [12 pp.]$$pReprod. fertil. dev.$$tREPRODUCTION FERTILITY AND DEVELOPMENT$$x1031-3613
000144590 8564_ $$s1628247$$uhttps://zaguan.unizar.es/record/144590/files/texto_completo.pdf$$yVersión publicada
000144590 8564_ $$s2595413$$uhttps://zaguan.unizar.es/record/144590/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000144590 909CO $$ooai:zaguan.unizar.es:144590$$particulos$$pdriver
000144590 951__ $$a2024-08-29-10:45:20
000144590 980__ $$aARTICLE