| Página principal > Artículos > Differential effect of melatonin on ram spermatozoa depending on the allelic variant of the RsaI polymorphism of the MTR1A gene, incubation medium and season > MARC |
000144590 001__ 144590 000144590 005__ 20250923084427.0 000144590 0247_ $$2doi$$a10.1071/RD23233 000144590 0248_ $$2sideral$$a139389 000144590 037__ $$aART-2024-139389 000144590 041__ $$aeng 000144590 100__ $$0(orcid)0000-0002-4670-3735$$aPeña-Delgado, Victoria$$uUniversidad de Zaragoza 000144590 245__ $$aDifferential effect of melatonin on ram spermatozoa depending on the allelic variant of the RsaI polymorphism of the MTR1A gene, incubation medium and season 000144590 260__ $$c2024 000144590 5060_ $$aAccess copy available to the general public$$fUnrestricted 000144590 5203_ $$aContext The RsaI polymorphism of the melatonin receptor MTNR1A gene affects seasonal reproduction in sheep, but its effect on ram spermatozoa and their response to melatonin is unknown. Aims This study aims to evaluate whether RsaI polymorphism of the MTNR1A gene influences the response of ram spermatozoa to in vitro added melatonin. Methods Spermatozoa from rams carrying different RsaI allelic variants were incubated with melatonin in a TALP medium or a capacitation-triggering medium during the reproductive and non-reproductive seasons. After incubation, sperm motility, membrane integrity, mitochondria activity, oxidative damage, apoptotic markers and capacitation status were assessed. Key results In the reproductive season, the T/T genotype was related to some adverse effects of melatonin when spermatozoa were incubated in TALP medium, whereas the C/C genotype was linked with adverse effects when the hormone was added in a capacitation-triggering medium. The decapacitating effect of melatonin on spermatozoa was also different depending on genotype. Conclusions The melatonin effect on spermatozoa from rams carrying different RsaI genotypes differed depending on the season and the medium. Implications The knowledge of the RsaI allelic variant of the MTNR1A gene of rams could be helpful when carrying out in vitro reproductive techniques in the ovine species. 000144590 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A07-23R$$9info:eu-repo/grantAgreement/ES/MICINN/PID2020-113239RB-I00 000144590 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc-nd$$uhttp://creativecommons.org/licenses/by-nc-nd/3.0/es/ 000144590 590__ $$a2.1$$b2024 000144590 592__ $$a0.327$$b2024 000144590 591__ $$aZOOLOGY$$b24 / 181 = 0.133$$c2024$$dQ1$$eT1 000144590 591__ $$aDEVELOPMENTAL BIOLOGY$$b20 / 39 = 0.513$$c2024$$dQ3$$eT2 000144590 591__ $$aREPRODUCTIVE BIOLOGY$$b27 / 42 = 0.643$$c2024$$dQ3$$eT2 000144590 593__ $$aBiotechnology$$c2024$$dQ3 000144590 593__ $$aAnimal Science and Zoology$$c2024$$dQ3 000144590 593__ $$aReproductive Medicine$$c2024$$dQ3 000144590 593__ $$aMolecular Biology$$c2024$$dQ4 000144590 593__ $$aGenetics$$c2024$$dQ4 000144590 593__ $$aEndocrinology$$c2024$$dQ4 000144590 593__ $$aDevelopmental Biology$$c2024$$dQ4 000144590 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000144590 700__ $$0(orcid)0000-0002-9552-4709$$aNoya, Agustí$$uUniversidad de Zaragoza 000144590 700__ $$0(orcid)0000-0003-3929-5064$$aCarvajal-Serna, Melissa$$uUniversidad de Zaragoza 000144590 700__ $$0(orcid)0000-0003-2827-3054$$aAbecia, José A.$$uUniversidad de Zaragoza 000144590 700__ $$0(orcid)0000-0002-2312-6402$$aPérez-Pe, Rosaura$$uUniversidad de Zaragoza 000144590 700__ $$0(orcid)0000-0003-1997-4262$$aCasao, Adriana$$uUniversidad de Zaragoza 000144590 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología 000144590 7102_ $$12008$$2700$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Producción Animal 000144590 773__ $$g36, 10 (2024), [12 pp.]$$pReprod. fertil. dev.$$tREPRODUCTION FERTILITY AND DEVELOPMENT$$x1031-3613 000144590 8564_ $$s1628247$$uhttps://zaguan.unizar.es/record/144590/files/texto_completo.pdf$$yVersión publicada 000144590 8564_ $$s2595413$$uhttps://zaguan.unizar.es/record/144590/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000144590 909CO $$ooai:zaguan.unizar.es:144590$$particulos$$pdriver 000144590 951__ $$a2025-09-22-14:40:21 000144590 980__ $$aARTICLE
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