An agent-based method to estimate 3D cell migration trajectories from 2D measurements: Quantifying and comparing T vs CAR-T 3D cell migration

Camacho-Gómez, Daniel (Universidad de Zaragoza) ; Movilla, Nieves ; Borau, Carlos ; Martín, Alejandro (Universidad de Zaragoza) ; Oñate Salafranca, Carmen ; Pardo, Julián (Universidad de Zaragoza) ; Gómez-Benito, María José (Universidad de Zaragoza) ; García-Aznar, José Manuel (Universidad de Zaragoza)

Resumen: Background and objective: Immune cell migration is one of the key features that enable immune cells to find invading pathogens, control tissue damage, and eliminate primary developing tumors. Chimeric antigen receptor (CAR) T-cell therapy is a novel strategy in the battle against various cancers. It has been successful in treating hematological tumors, yet it still faces many challenges in the case of solid tumors. In this work, we evaluate the three-dimensional (3D) migration capacity of T and CAR-T cells within dense collagen-based hydrogels. Quantifying three-dimensional (3D) cell migration requires microscopy techniques that may not be readily accessible. Thus, we introduce a straightforward mathematical model designed to infer 3D trajectories of cells from two-dimensional (2D) cell trajectories.
Methods: We develop a 3D agent-based model (ABM) that simulates the temporal changes in the direction of migration with an inverse transform sampling method. Then, we propose an optimization procedure to accurately orient cell migration over time to reproduce cell migration from 2D experimental cell trajectories. With this model, we simulate cell migration assays of T and CAR-T cells in microfluidic devices conducted under hydrogels with different concentrations of type I collagen and validate our 3D cell migration predictions with light-sheet microscopy.
Results: Our findings indicate that CAR-T cell migration is more sensitive to collagen concentration increases than T cells, resulting in a more pronounced reduction in their invasiveness. Moreover, our computational model reveals significant differences in 3D movement patterns between T and CAR-T cells. T cells exhibit migratory behavior in 3D whereas that CAR-T cells predominantly move within the plane, with limited movement in the direction. However, upon the introduction of a CXCL12 chemical gradient, CAR-T cells present migration patterns that closely resemble those of T cells.
Conclusions: This framework demonstrates that 2D projections of 3D trajectories may not accurately represent real migration patterns. Moreover, it offers a tool to estimate 3D migration patterns from 2D experimental data, which can be easily obtained with automatic quantification algorithms. This approach helps reduce the need for sophisticated and expensive microscopy equipment required in laboratories, as well as the computational burden involved in producing and analyzing 3D experimental data.

Idioma: Inglés

DOI: 10.1016/j.cmpb.2024.108331

Año: 2024

Publicado en: Computer Methods and Programs in Biomedicine 255 (2024), 108331 [9 pp.]
ISSN: 0169-2607

Factor impacto JCR: 4.8 (2024)

Categ. JCR: COMPUTER SCIENCE, THEORY & METHODS rank: 20 / 147 = 0.136 (2024) - Q1 - T1
Categ. JCR: MEDICAL INFORMATICS rank: 11 / 48 = 0.229 (2024) - Q1 - T1
Categ. JCR: ENGINEERING, BIOMEDICAL rank: 35 / 124 = 0.282 (2024) - Q2 - T1
Categ. JCR: COMPUTER SCIENCE, INTERDISCIPLINARY APPLICATIONS rank: 47 / 175 = 0.269 (2024) - Q2 - T1

Factor impacto SCIMAGO: 1.13 - Computer Science Applications (Q1) - Software (Q1) - Health Informatics (Q1)

Financiación: info:eu-repo/grantAgreement/ES/AEI/PID2020-113963RB-I00
Financiación: info:eu-repo/grantAgreement/ES/DGA/B29-20R
Financiación: info:eu-repo/grantAgreement/EC/H2020/101018587/EU/Individual and Collective Migration of the Immune Cellular System/ICoMICS
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/CB21-13-00087
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/FORT23-00028
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PLEC2021-007709/AEI/10.13039/501100011033

Tipo y forma: Article (Published version)

Área (Departamento): Área Inmunología (Dpto. Microb.Ped.Radio.Sal.Pú.)
Área (Departamento): Área Mec.Med.Cont. y Teor.Est. (Dpto. Ingeniería Mecánica)