Colorimetric enzymatic rapid test for the determination of atropine in baby food using a smartphone

Domínguez, M. (Universidad de Zaragoza) ; Moraru, D. ; Lasso, S. ; Sanz-Vicente, I. (Universidad de Zaragoza) ; Marcos, S. de (Universidad de Zaragoza) ; Galbán, J. (Universidad de Zaragoza)

Resumen: A method for the enzymatic determination of atropine has been developed, which is based on a sequence of reactions involving (1) the hydrolysis of atropine to give tropine; (2) the enzymatic oxidation of tropine with NAD (catalysed by tropinone reductase); and (3) an indicator reaction, in which the NADH previously formed reduces the dye iodonitrotetrazolium chloride (INT) to a reddish species, the reaction catalysed by diaphorase. The method was first developed in solution (linear response range from 2.4 × 10−6 M to 1.0 × 10−4 M). It was then implemented in cellulose platforms to develop a rapid test where the determination is made by measuring the RGB coordinates of the platforms using a smartphone-based device. The device is based on the integrating sphere concept and contains a light source to avoid external illumination effects. The smartphone is controlled by an app that allows a calibration line to be generated and the atropine concentration to be quantified; moreover, since the app normalizes the CCD response of the smartphone, the results and calibrations obtained with different smartphones are similar and can be shared. Using the G coordinate, the results were shown to have a linear response with the concentration of atropine ranging from 1.2 × 10−5 M to 3.0 × 10−4 M with an RSD of 1.4% (n = 5). The method has been applied to the determination of atropine in baby food and buckwheat samples with good results.

Idioma: Inglés

DOI: 10.1007/s00216-024-05401-x

Año: 2024

Publicado en: Analytical and Bioanalytical Chemistry 416 (2024), 7317–7323
ISSN: 1618-2642

Financiación: info:eu-repo/grantAgreement/ES/DGA/E25-23R
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2019-105408GB-I00
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2022-139235OB-I00

Tipo y forma: Article (Published version)

Área (Departamento): Área Química Analítica (Dpto. Química Analítica)