Membrane-localized magnetic hyperthermia promotes intracellular delivery of cell-impermeant probes

Idiago-López, Javier ; Ferreira, Daniela ; Asín, Laura (Universidad de Zaragoza) ; Moros, María ; Armenia, Ilaria ; Grazú, Valeria ; Fernandes, Alexandra R. ; de la Fuente, Jesús M. ; Baptista, Pedro V. ; Fratila, Raluca M.

Resumen: In this work, we report the disruptive use of membrane-localized magnetic hyperthermia to promote the internalization of cell-impermeant probes. Under an alternating magnetic field, magnetic nanoparticles (MNPs) immobilized on the cell membrane via bioorthogonal click chemistry act as nanoheaters and lead to the thermal disruption of the plasma membrane, which can be used for internalization of different types of molecules, such as small fluorescent probes and nucleic acids. Noteworthily, no cell death, oxidative stress and alterations of the cell cycle are detected after the thermal stimulus, although cells are able to sense and respond to the thermal stimulus through the expression of different types of heat shock proteins (HSPs). Finally, we demonstrate the utility of this approach for the transfection of cells with a small interference RNA (siRNA), revealing a similar efficacy to a standard transfection method based on the use of cationic lipid-based reagents (such as Lipofectamine), but with lower cell toxicity. These results open the possibility of developing new procedures for “opening and closing” cellular membranes with minimal disturbance of cellular integrity. This on-demand modification of cell membrane permeability could allow the direct intracellular delivery of biologically relevant (bio)molecules, drugs and nanomaterials, thus overcoming traditional endocytosis pathways and avoiding endosomal entrapment.

Idioma: Inglés

DOI: 10.1039/d4nr01955e

Año: 2024

Publicado en: Nanoscale 16, 32 (2024), 15176-15195
ISSN: 2040-3364

Financiación: info:eu-repo/grantAgreement/ES/DGA/E15-23R
Financiación: info:eu-repo/grantAgreement/ES/MCIU-AEI-FEDER/BIO2017-84246-C2-1-R
Financiación: info:eu-repo/grantAgreement/ES/MCIU/CAS18-00233
Financiación: info:eu-repo/grantAgreement/ES/MCIU/CNS2023-144436
Financiación: info:eu-repo/grantAgreement/ES/MCIU/PCIN-2017-060
Financiación: info:eu-repo/grantAgreement/ES/MCIU/PGC2018-096016-B-I00
Financiación: info:eu-repo/grantAgreement/ES/MCIU/RYC-2019-026860-I

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)