Resumen: Purpose: The purpose of this study was to evaluate the in vivo efficacy of the estimations of wavefront analyzers using Hartmann–Shack technology to measure optical aberrations when the pupil size is smaller than the evaluated pupil area. Methods: Patients implanted with the monofocal ZCB00 intraocular lens (Johnson and Johnson) were examined with the KR-1W Wavefront Analyzer (Topcon) without pharmacological mydriasis and with it afterward. Optical aberrations were analyzed considering a 4-mm pupil and a 6-mm pupil for both examinations. Results: Sixty-six eyes of 33 patients with a mean axial length of 23.35 ± 0.91 mm were assessed. The mean pupil diameter at the baseline examination was 5.05 ± 0.88 mm and under pharmacological mydriasis, it was 6.29 ± 0.84 mm. Outcomes were similar with and without dilation in the 4-mm comparison. However, there was a great disparity in the 6-mm comparison. Most of the values obtained under mydriasis were statistically lower than at baseline (P < 0.05). Conclusion: The iris interferes with measurements of wavefront aberrations, and therefore, real pupil size should always be checked before evaluating optical aberrations with Hartman–Shack sensors. When pupil size is smaller than the analyzed diameter, ocular, and internal, and sometimes, corneal aberrations are estimated far more positive than real values. Idioma: Inglés DOI: 10.4103/sjopt.sjopt_123_23 Año: 2024 Publicado en: Saudi Journal of Ophthalmology 38, 2 (2024), 168-172 ISSN: 1319-4534 Factor impacto SCIMAGO: 0.408 - Ophthalmology (Q3)