000145730 001__ 145730
000145730 005__ 20241122130824.0
000145730 0247_ $$2doi$$a10.3390/vetsci11090420
000145730 0248_ $$2sideral$$a140686
000145730 037__ $$aART-2024-140686
000145730 041__ $$aeng
000145730 100__ $$0(orcid)0000-0003-0770-9250$$aAusejo-Marcos, Raquel$$uUniversidad de Zaragoza
000145730 245__ $$aSpermiogram, Kinetics, Flow Cytometric Characteristics and DNA Damage Degree in Boar Ejaculates: Summarization and Clustering
000145730 260__ $$c2024
000145730 5060_ $$aAccess copy available to the general public$$fUnrestricted
000145730 5203_ $$aBoar semen analysis includes sperm motility, concentration, morphology and other more complex analyses such as membrane integrity, DNA damage and seminal plasma components. This study aims to summarize these numerous data by linear combinations of them, to classify ejaculates in several categories (clusters) and to investigate the potential differences among clusters on fertility and prolificacy. Young Pietrain boars (23 ± 3.6 months) were investigated: ten boars from the Nucléus genetic line (group 1: 90 ejaculates weekly) and five boars from the Batallé genetic line (group 2: 30 ejaculates weekly). Computer-assisted semen analysis (CASA) examined motility. Sperm viability, acrosome reaction, early apoptosis, mitochondrial activity and DNA damage were studied by flow cytometry analysis. SPSS v.26 software was used to perform principal component analysis (PCA) and clustering. Three principal components (PC1: speed; PC2: linear path; PC3: DNA damage) were detected and four clusters identified in both groups. Clusters also differed significantly in several variables not included in these PCs (group 1: beat cross frequency and poly (ADP-ribose) polymerase; group 2: cathepsin B, abnormal forms, mitochondrial activity and high DNA stainability). PCA and clustering achieved adequate description of these ejaculates, but no differences among clusters were found for fertility or prolificacy, probably because the minimum sperm requirements had been met.
000145730 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000145730 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000145730 700__ $$0(orcid)0000-0001-5026-5144$$aTejedor, María Teresa$$uUniversidad de Zaragoza
000145730 700__ $$0(orcid)0000-0001-6733-9373$$aMiguel-Jiménez, Sara
000145730 700__ $$aGómez-Giménez, Belén
000145730 700__ $$aSoriano-Úbeda, Cristina
000145730 700__ $$aMendoza, Noelia
000145730 700__ $$aVicente-Carrillo, Alejandro
000145730 700__ $$aHurtado, William Fernando
000145730 700__ $$aÁvila Holguín, Celia
000145730 700__ $$0(orcid)0000-0001-6629-6842$$aMoreno, Bernardino$$uUniversidad de Zaragoza
000145730 700__ $$0(orcid)0000-0001-6964-7011$$aFalceto, María Victoria$$uUniversidad de Zaragoza
000145730 7102_ $$11001$$2420$$aUniversidad de Zaragoza$$bDpto. Anatom.,Embri.Genét.Ani.$$cÁrea Genética
000145730 7102_ $$11009$$2773$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Sanidad Animal
000145730 7102_ $$11009$$2617$$aUniversidad de Zaragoza$$bDpto. Patología Animal$$cÁrea Medicina y Cirugía Animal
000145730 773__ $$g11, 9 (2024), 420 [17 pp.]$$tVeterinary Sciences$$x2306-7381
000145730 8564_ $$s324564$$uhttps://zaguan.unizar.es/record/145730/files/texto_completo.pdf$$yVersión publicada
000145730 8564_ $$s2902772$$uhttps://zaguan.unizar.es/record/145730/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000145730 909CO $$ooai:zaguan.unizar.es:145730$$particulos$$pdriver
000145730 951__ $$a2024-11-22-11:53:26
000145730 980__ $$aARTICLE