Dissecting the molecular mechanism of apoptosis during photothermal therapy using gold nanoprisms

Pérez-Hernández, M. ; Del Pino, P. ; Mitchell, S. G. ; Moros, M. ; Stepien, G. ; Pelaz, B. ; Parak, W. J. ; Gálvez, E. M. ; Pardo, J. (Universidad de Zaragoza) ; De La Fuente, J.M. (Universidad de Zaragoza)

Resumen: The photothermal response of plasmonic nanomaterials can be exploited for a number of biomedical applications in diagnostics (biosensing and optoacoustic imaging) and therapy (drug delivery and photothermal therapy). The most common cellular response to photothermal cancer treatment (ablation of solid tumors) using plasmonic nanomaterials is necrosis, a process that releases intracellular constituents into the extracellular milieu producing detrimental inflammatory responses. Here we report the use of laser-induced photothermal therapy employing gold nanoprisms (NPRs) to specifically induce apoptosis in mouse embryonic fibroblast cells transformed with the SV40 virus. Laser-irradiated "hot" NPRs activate the intrinsic/mitochondrial pathway of apoptosis (programmed cell death), which is mediated by the nuclear-encoded proteins Bak and Bax through the activation of the BH3-only protein Bid. We confirm that an apoptosis mechanism is responsible by showing how the NPR-mediated cell death is dependent on the presence of caspase-9 and caspase-3 proteins. The ability to selectively induce apoptotic cell death and to understand the subsequent mechanisms provides the foundations to predict and optimize NP-based photothermal therapy to treat cancer patients suffering from chemo- and radioresistance

Idioma: Inglés

DOI: 10.1021/nn505468v

Año: 2015

Publicado en: ACS NANO 9, 1 (2015), 52-61
ISSN: 1936-0851

Factor impacto JCR: 13.334 (2015)

Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 8 / 162 = 0.049 (2015) - Q1 - T1
Categ. JCR: MATERIALS SCIENCE, MULTIDISCIPLINARY rank: 9 / 270 = 0.033 (2015) - Q1 - T1
Categ. JCR: CHEMISTRY, PHYSICAL rank: 6 / 144 = 0.042 (2015) - Q1 - T1
Categ. JCR: NANOSCIENCE & NANOTECHNOLOGY rank: 4 / 82 = 0.049 (2015) - Q1 - T1

Factor impacto SCIMAGO: 6.712 - Engineering (miscellaneous) (Q1) - Physics and Astronomy (miscellaneous) (Q1) - Nanoscience and Nanotechnology (Q1) - Materials Science (miscellaneous) (Q1)

Financiación: info:eu-repo/grantAgreement/ES/MINECO/MAT2011-26851-CO2-01
Financiación: info:eu-repo/grantAgreement/ES/MINECO/MAT2011-26851-CO2-02

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Inmunología (Dpto. Microb.Med.Pr.,Sal.Públ.)
Área (Departamento): Área Química Orgánica (Dpto. Química Orgánica)