Jaagsiekte sheep retrovirus found in milk macrophages but not in milk lymphocytes or mammary gland epithelia of naturally infected sheep

Borobia, Marta (Universidad de Zaragoza) ; De las Heras, Marcelo (Universidad de Zaragoza) ; Godino, Javier ; Ferrer, Luis M. (Universidad de Zaragoza) ; Lacasta, Delia (Universidad de Zaragoza) ; Loste, Araceli (Universidad de Zaragoza) ; Ramos, Juan J. (Universidad de Zaragoza) ; Ortín, Aurora (Universidad de Zaragoza)

Resumen: Jaagsiekte sheep retrovirus (JSRV) causes ovine pulmonary adenocarcinoma. JSRV can be transmitted via infected colostrum or milk, which contain somatic cells (SCs) harboring JSRV provirus. Nevertheless, the cell types involved in this form of transmission and the involvement of the mammary gland remain unknown. We separated adherent cells (macrophages and monocytes) by plastic adherence, and lymphocytes (CD4+ and CD8+ T cells, and B cells) by flow cytometry, from SCs in milk samples from 12 naturally infected, PCR blood test JSRV-positive, subclinical ewes. These cell populations were tested by PCR to detect JSRV provirus. The ewes were euthanized, and mammary gland samples were analyzed immunohistochemically to detect JSRV surface protein. We did not detect JSRV provirus in any milk lymphocyte population, but milk adherent cells were positive in 3 of 12 sheep, suggesting a potential major role of this population in the lactogenic transmission of JSRV. Immunohistochemistry did not reveal positive results in mammary epithelial cells, pointing to a lack of participation of the mammary gland in the biological cycle of JSRV and reducing the probability of excretion of free viral particles in colostrum or milk.

Idioma: Inglés

DOI: 10.1177/10406387211039196

Año: 2021

Publicado en: JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION 78 (2021), 104063872110391
ISSN: 1040-6387

Factor impacto JCR: 1.569 (2021)

Categ. JCR: VETERINARY SCIENCES rank: 74 / 145 = 0.51 (2021) - Q3 - T2

Factor impacto CITESCORE: 2.3 - Veterinary (Q2)

Factor impacto SCIMAGO: 0.457 - Veterinary (miscellaneous) (Q2)

Financiación: info:eu-repo/grantAgreement/ES/MICINN/AGL2008-00803

Tipo y forma: Article (Published version)

Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)
Área (Departamento): Área Medicina y Cirugía Animal (Dpto. Patología Animal)