Antibiotic-eluting orthopedic device to prevent early implant associated infections: Efficacy, biocompatibility and biodistribution studies in an ovine model

Gimeno, María ; Pinczowski, Pedro ; Mendoza, Gracia ; Asín, Javier (Universidad de Zaragoza) ; Vázquez, Francisco J. (Universidad de Zaragoza) ; Vispe, Eugenio ; García-Álvarez, Felícito (Universidad de Zaragoza) ; Pérez, Marta (Universidad de Zaragoza) ; Santamaría, Jesús (Universidad de Zaragoza) ; Arruebo, Manuel (Universidad de Zaragoza) ; Luján, Lluís (Universidad de Zaragoza)

Resumen: Infection of orthopedic devices is a major complication in the postsurgical period generating important health issues and economic consequences. Prevention strategies could be based on local release of antibiotics from the orthopedic device itself to avoid adhesion and growth of bacteria. The purpose of this work is to demonstrate the efficiency to prevent these infections by a cefazolin-eluting, perforated stainless steel implant in an in vivo ovine model. The device was placed in the tibia of sheep, one group receiving cefazolin-loaded implants whereas the control group received empty implants. All implants were experimentally infected by direct inoculation of Staphylococcus aureus ATCC 6538. In vitro cytotoxicological studies were also performed to check the effect of antibiotic on cell viability, integrity, and cycle. Results showed that sheep receiving cefazolin-loaded devices were able to avoid implant-associated infections, with normal tissue healing process. The antibiotic release followed a local concentric pattern as demonstrated by high-performance liquid chromatography detection in tissues. The in vitro results indicate the lack of relevant cytotoxic effects for the maximum antibiotic concentration released by the device. These results demonstrate the efficiency and safety of cefazolin-eluting implants in an ovine model to prevent early postsurgical infections of orthopedic devices

Idioma: Inglés

DOI: 10.1002/jbm.b.34009

Año: 2018

Publicado en: Journal of Biomedical Materials Research - Part B Applied Biomaterials 106 , 5 (2018), 1976-1986
ISSN: 1552-4973

Factor impacto JCR: 2.674 (2018)

Categ. JCR: ENGINEERING, BIOMEDICAL rank: 29 / 80 = 0.363 (2018) - Q2 - T2
Categ. JCR: MATERIALS SCIENCE, BIOMATERIALS rank: 19 / 32 = 0.594 (2018) - Q3 - T2

Factor impacto SCIMAGO: 0.658 - Biomedical Engineering (Q2) - Biomaterials (Q2)

Financiación: info:eu-repo/grantAgreement/EC/FP7/614715/EU/A Photo-triggered On-demand Drug Delivery System for Chronic Pain/NANOHEDONISM

Tipo y forma: Article (PostPrint)

Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)
Área (Departamento): Área Anatom.Anatom.Patológ.Com (Dpto. Anatom.,Embri.Genét.Ani.)
Área (Departamento): Área Traumatología y Ortopedia (Dpto. Cirugía,Ginecol.Obstetr.)
Área (Departamento): Área Ingeniería Química (Dpto. Ing.Quím.Tecnol.Med.Amb.)
Área (Departamento): Área Medicina y Cirugía Animal (Dpto. Patología Animal)