Design of a Magnetic Nanoplatform Based on CD26 Targeting and HSP90 Inhibition for Apoptosis and Ferroptosis-Mediated Elimination of Senescent Cells

Wnuk, Maciej ; Del Sol-Fernández, Susel ; Bloniarz, Dominika ; Slaby, Julia ; Szmatola, Tomasz ; Zebrowski, Michal ; Martínez-Vicente, Pablo (Universidad de Zaragoza) ; Litwinienko, Grzegorz ; Moros, María ; Lewinska, Anna

Resumen: The accumulation of senescent cells, a hallmark of aging and age-related diseases, is also considered as a side effect of anticancer therapies, promoting drug resistance and leading to treatment failure. The use of senolytics, selective inducers of cell death in senescent cells, is a promising pharmacological antiaging and anticancer approach. However, more studies are needed to overcome the limitations of first-generation senolytics by the design of targeted senolytics and nanosenolytics and the validation of their usefulness in biological systems. In the present study, we have designed a nanoplatform composed of iron oxide nanoparticles functionalized with an antibody against a cell surface marker of senescent cells (CD26), and loaded with the senolytic drug HSP90 inhibitor 17-DMAG (MNP@CD26@17D). We have documented its action against oxidative stress-induced senescent human fibroblasts, WI-38 and BJ cells, and anticancer drug-induced senescent cutaneous squamous cell carcinoma A431 cells, demonstrating for the first time that CD26 is a valid marker of senescence in cancer cells. A dual response to MNP@CD26@17D stimulation in senescent cells was revealed, namely, apoptosis-based early response (2 h treatment) and ferroptosis-based late response (24 h treatment). MNP@CD26@17D-mediated ferroptosis might be executed by ferritinophagy as judged by elevated levels of the ferritinophagy marker NCOA4 and a decreased pool of ferritin. As 24 h treatment with MNP@CD26@17D did not induce hemolysis in human erythrocytes in vitro, this newly designed nanoplatform could be considered as an optimal multifunctional tool to target and eliminate senescent cells of skin origin, overcoming their apoptosis resistance.

Idioma: Inglés

DOI: 10.1021/acsbiomaterials.4c00771

Año: 2024

Publicado en: ACS BIOMATERIALS SCIENCE & ENGINEERING 11, 1 (2024), 280-297
ISSN: 2373-9878

Financiación: info:eu-repo/grantAgreement/ES/DGA/E15-23R
Financiación: info:eu-repo/grantAgreement/EC/HORIZON EUROPE/101064735/EU/Tunning the force for remote magnetomechanical gating of Piezo1 channels/MAGPIEZ
Financiación: info:eu-repo/grantAgreement/EC/H2020/853468/EU/Remote control of cellular signalling triggered by magnetic switching/SIROCCO
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2021-122508NB-I00
Financiación: info:eu-repo/grantAgreement/ES/MICIU/PCI2023-143448

Tipo y forma: Article (Published version)

Área (Departamento): Área Toxicología (Dpto. Bioq.Biolog.Mol. Celular)