Automatic quantification of myocardial remodeling features in human ventricular tissue from label-free microscopy

García-Mendívil, Laura (Universidad de Zaragoza) ; Pérez-Zabalza, María ; Duwé, Sam ; Ordovás, Laura ; Pueyo, Esther (Universidad de Zaragoza)

Resumen: The procedures used routinely for collagen and lipofuscin evaluation are, in many cases, qualitative, observer dependent, and disregard spatial distribution. Here, we present a protocol for automatic quantification and spatial characterization of collagen and lipofuscin from label-free microscopy images of human ventricular tissues. We describe the steps for sample collection, tissue processing, image acquisition, and quantification of collagen and lipofuscin. This protocol avoids discrepancies between observers and can be adapted to other tissues and species.

For complete details on the use and execution of this protocol, please refer to García-Mendívil et al. (2022).

Idioma: Inglés

DOI: 10.1016/j.xpro.2023.102730

Año: 2023

Publicado en: STAR Protocols 4, 4 (2023), 102730 [14 pp.]
ISSN: 2666-1667

Factor impacto CITESCORE: 2.0 - Biochemistry, Genetics and Molecular Biology (all) (Q3) - Immunology and Microbiology (all) (Q3) - Neuroscience (all) (Q4)

Factor impacto SCIMAGO: 0.955 - Immunology and Microbiology (miscellaneous) (Q1) - Neuroscience (miscellaneous) (Q2) - Biochemistry, Genetics and Molecular Biology (miscellaneous) (Q2)

Tipo y forma: Article (Published version)

Área (Departamento): Área Teoría Señal y Comunicac. (Dpto. Ingeniería Electrón.Com.)