A Novel Multicompartment Barrier-Free Microfluidic Device Reveals the Impact of Extracellular Matrix Stiffening and Temozolomide on Immune-Tumor Interactions in Glioblastoma
Bayona, Clara ; Olaizola-Rodrigo, Claudia ; Sharko, Vira ; Ashrafi, Mehran ; Barrio, Jesús del (Universidad de Zaragoza) ; Doblaré, Manuel (Universidad de Zaragoza) ; Monge, Rosa ; Ochoa, Ignacio (Universidad de Zaragoza) ; Oliván, Sara (Universidad de Zaragoza)
Resumen: The immune system plays a crucial role in shaping the glioblastoma tumor microenvironment, characterized by its complexity and dynamic interactions. Understanding the tumor-immune crosstalk is essential for advancing cancer research and therapeutic development. Here, a novel multicompartment, barrier-free microfluidic device is presented that overcomes the limitations of existing models by enabling direct tumor-immune interactions without physical barriers, preserving natural immune cell infiltration. This platform supports the independent and simultaneous culture of tumor and immune cells, replicating the healthy-tumoral stroma interface, and allows investigating the effect of matrix stiffness and chemotherapy on both populations. The findings reveal that increased collagen concentration promotes tumor invasiveness while impairing immune cell infiltration. Additionally, temozolomide treatment reduces immune cell motility but enhances anti-tumor immune responses. These insights highlight the critical roles of extracellular matrix mechanics and chemotherapy in tumor progression and immune modulation, establishing this device as a powerful tool for studying glioblastoma-immune dynamics and evaluating therapeutic strategies.
Idioma: Inglés
DOI: 10.1002/smll.202409229
Año: 2025
Publicado en: Small 21, 9 (2025), e2409229 [14 pp.]
ISSN: 1613-6810
Financiación: info:eu-repo/grantAgreement/ES/AEI/PID2021-126051OB-C41
Financiación: info:eu-repo/grantAgreement/ES/DGA/T62-230R
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/PT23-00146
Financiación: info:eu-repo/grantAgreement/ES/MINECO/DIN2020-011544
Tipo y forma: Article (Published version)
Área (Departamento): Area Histología (Dpto. Anatom.Histolog.Humanas)
Área (Departamento): Área Química Orgánica (Dpto. Química Orgánica)
Área (Departamento): Área Mec.Med.Cont. y Teor.Est. (Dpto. Ingeniería Mecánica)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes.
Exportado de SIDERAL (2025-10-17-14:31:30)
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Idioma: Inglés
DOI: 10.1002/smll.202409229
Año: 2025
Publicado en: Small 21, 9 (2025), e2409229 [14 pp.]
ISSN: 1613-6810
Financiación: info:eu-repo/grantAgreement/ES/AEI/PID2021-126051OB-C41
Financiación: info:eu-repo/grantAgreement/ES/DGA/T62-230R
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/PT23-00146
Financiación: info:eu-repo/grantAgreement/ES/MINECO/DIN2020-011544
Tipo y forma: Article (Published version)
Área (Departamento): Area Histología (Dpto. Anatom.Histolog.Humanas)
Área (Departamento): Área Química Orgánica (Dpto. Química Orgánica)
Área (Departamento): Área Mec.Med.Cont. y Teor.Est. (Dpto. Ingeniería Mecánica)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes. Exportado de SIDERAL (2025-10-17-14:31:30)
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Este artículo se encuentra en las siguientes colecciones:
Articles > Artículos por área > Mec. de Medios Contínuos y Teor. de Estructuras
Articles > Artículos por área > Química Orgánica
Articles > Artículos por área > Histología
Record created 2025-02-10, last modified 2025-10-17