Postprandial changes in gene expression of hepatic cholesterol metabolism in response to two protein sources in the rat

Allaoui, Amine ; Dehiba, Faiza ; Herrera Marcos, Luis Vicente (Universidad de Zaragoza) ; Rodríguez Yoldi, María Jesús (Universidad de Zaragoza) ; Osada, Jesús (Universidad de Zaragoza) ; Boualga, Ahmed

Resumen: A hypocholesterolemic effect of chickpea (CPH) and sardine protein hydrolysates (SPH) has been observed. Two main mechanisms could be involved in explaining this fact: the inhibition of cholesterol enterohepatic cycle and a post-absorptive regulatory pathway. We aimed to check whether these hypotheses were involved in the present study. Three groups of rats were given a single dose of a cholesterol-oil solution (HC), supplemented or not with CPH or SPH. The postprandial transcription levels of some genes involved in cholesterol metabolism were assessed in their livers. Four hours after feeding, the results showed that Mttp, Pltp, Cidec, Abca1, and Abcg1 gene expressions were similar among the different groups. Lcat mRNA was 5.5-fold higher in CPH and SPH rats’ liver vs. HC, but this difference was not statistically significant. SPH tends to upregulate Ldlr expression, while CPH tends to upregulate Cyp7a1 transcription (2- and 8-fold, respectively, compared to HC rats, p=0.083 according to the Mann-Whitney test). Pon1 and Apoa1 were not affected by the hydrolysate supplementation vs. HC group. In conclusion, these results suggest that chickpeas and sardine protein hydrolysates exert hypocholesterolemic activity mainly by enterohepatic inhibiting the cholesterol cycle rather than modulating the postprandial gene expressions involved in cholesterol metabolism.

Idioma: Inglés

DOI: 10.46389/rjd-2024-1441

Año: 2024

Publicado en: Romanian Journal of Diabetes, Nutrition and Metabolic Diseases 31, 2 (2024), 143-149
ISSN: 1583-8609

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Fisiología (Dpto. Farmac.Fisiol.y Med.L.F.)
Área (Departamento): Area Anatom.Embriol.Humana (Dpto. Anatom.Histolog.Humanas)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)