NleB/SseK-catalyzed arginine-glycosylation and enteropathogen virulence are finely tuned by a single variable position contiguous to the catalytic machinery
García-García A. (Universidad de Zaragoza) ; Hicks T. ; El Qaidi S. ; Zhu C. ; Hardwidge P.R. ; Angulo J. ; Hurtado-Guerrero R.
Resumen: NleB/SseK effectors are arginine-GlcNAc-transferases expressed by enteric bacterial pathogens that modify host cell proteins to disrupt signaling pathways. While the conservedCitrobacter rodentiumNleB andE. coliNleB1 proteins display a broad selectivity towards host proteins, Salmonella entericaSseK1, SseK2, and SseK3 have a narrowed protein substrate selectivity. Here, by combining computational and biophysical experiments, we demonstrate that the broad protein substrate selectivity of NleB relies on Tyr284NleB/NleB1, a second-shell residue contiguous to the catalytic machinery. Tyr284NleB/NleB1is important in coupling protein substrate binding to catalysis. This is exemplified by S286YSseK1and N302YSseK2mutants, which become active towards FADD and DR3 death domains, respectively, and whose kinetic properties match those of enterohemorrhagicE. coliNleB1. The integration of these mutants intoS. entericaincreasesS. entericasurvival in macrophages, suggesting that better enzymatic kinetic parameters lead to enhanced virulence. Our findings provide insights into how these enzymes finely tune arginine-glycosylation and, in turn, bacterial virulence. In addition, our data show how promiscuous glycosyltransferases preferentially glycosylate specific protein substrates.
Idioma: Inglés
DOI: 10.1039/d1sc04065k
Año: 2021
Publicado en: CHEMICAL SCIENCE 12, 36 (2021), 12181-12191
ISSN: 2041-6520
Factor impacto JCR: 9.969 (2021)
Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 25 / 179 = 0.14 (2021) - Q1 - T1
Factor impacto SCIMAGO: 2.878 - Chemistry (miscellaneous) (Q1)
Financiación: info:eu-repo/grantAgreement/ES/DGA-FEDER/E34-R17
Financiación: info:eu-repo/grantAgreement/ES/DGA/LMP58-18
Financiación: info:eu-repo/grantAgreement/ES/MICINN/BFU2016-75633-P
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2019-105451GB-I00
Tipo y forma: Article (Published version)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes.
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Idioma: Inglés
DOI: 10.1039/d1sc04065k
Año: 2021
Publicado en: CHEMICAL SCIENCE 12, 36 (2021), 12181-12191
ISSN: 2041-6520
Factor impacto JCR: 9.969 (2021)
Categ. JCR: CHEMISTRY, MULTIDISCIPLINARY rank: 25 / 179 = 0.14 (2021) - Q1 - T1
Factor impacto SCIMAGO: 2.878 - Chemistry (miscellaneous) (Q1)
Financiación: info:eu-repo/grantAgreement/ES/DGA-FEDER/E34-R17
Financiación: info:eu-repo/grantAgreement/ES/DGA/LMP58-18
Financiación: info:eu-repo/grantAgreement/ES/MICINN/BFU2016-75633-P
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2019-105451GB-I00
Tipo y forma: Article (Published version)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes. Exportado de SIDERAL (2025-10-17-14:15:54)
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Record created 2025-02-27, last modified 2025-10-17