Effect of L-Hyp supplementation on collagen muscle histology, gene expression, growth performance, body composition and fillet texture on big size European sea bass (Dicentrarchux labrax)

Castro P.L. ; Plasencia S. ; Zamorano M.J. ; Guerrero L. ; Claret A. ; Beltrán Gracia J.A. (Universidad de Zaragoza) ; Calanche Morales J. (Universidad de Zaragoza) ; Ginés R.

Resumen: Hydroxyproline (L-Hyp) is amply present in fishmeal but limited in plant-protein sources. This study was conducted to evaluate the effects of supplementation with dietary L-Hyp on the distribution of collagen types in the muscle, and on the texture, survival rate, growth rate, feed utilization, body composition as well as the expression of the gene that encodes the pro-alpha2 chains of type I collagen (Col Ia2) of large European sea bass (initial body weight 609.21 ± 75.39 g) from high plant-protein diets. Four isoproteic (42 % crude protein) and isolipidic (20 % crude lipid) experimental diets were formulated adding 0.6 (HL diet), 1.2 (HM diet), and 2% (HH diet) L-Hyp, respectively. Three periods of feeding of 45, 99 and 143 days were studied. L-Hyp supplementation at 1.2 % and 2 % significantly improved specific growth rate (SGR) and feed conversion ratio (FCR). In the white muscle, type I, IV collagen and trichromic stain were significantly higher in HH feed than the control diet. In the red muscle, only type I collagen was higher. HH diet, also increases Col Ia2 mRNA levels in muscle significantly. It can be concluded that the addition of crystalline L-Hyp at 1.2 % or 2 % in high plant-protein diets indicates positive effects on growth performance of adult European sea bass and increase in muscle total collagen deposition. © 2021 The Author(s)

Idioma: Inglés

DOI: 10.1016/j.aqrep.2021.100787

Año: 2021

Publicado en: Aquaculture Reports 21 (2021), 100787 [9 pp.]
ISSN: 2352-5134

Factor impacto JCR: 3.385 (2021)

Categ. JCR: FISHERIES rank: 13 / 54 = 0.241 (2021) - Q1 - T1

Factor impacto CITESCORE: 2.8 - Agricultural and Biological Sciences (Q2)

Factor impacto SCIMAGO: 0.613 - Aquatic Science (Q1) - Animal Science and Zoology (Q1)

Financiación: info:eu-repo/grantAgreement/ES/MINECO/RTA2013-00075-C03-02

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Tecnología de Alimentos (Dpto. Produc.Animal Cienc.Ali.)