000152081 001__ 152081
000152081 005__ 20250326144155.0
000152081 0247_ $$2doi$$a10.1128/aem.01829-24
000152081 0248_ $$2sideral$$a143394
000152081 037__ $$aART-2025-143394
000152081 041__ $$aeng
000152081 100__ $$aIvanova, Mirena
000152081 245__ $$aLarge-scale phenotypic and genomic analysis of Listeria monocytogenes reveals diversity in the sensitivity to quaternary ammonium compounds but not to peracetic acid
000152081 260__ $$c2025
000152081 5060_ $$aAccess copy available to the general public$$fUnrestricted
000152081 5203_ $$aListeria monocytogenes presents a significant concern for the food industry due to its ability to persist in the food processing environment. One of the factors contributing to its persistence is decreased sensitivity to disinfectants. Our objective was to assess the diversity of L. monocytogenes sensitivity to food industry disinfectants by testing the response of 1,671 L. monocytogenes isolates to quaternary ammonium compounds (QACs) and 414 isolates to peracetic acid (PAA) using broth microdilution and growth curve analysis assays, respectively, and to categorize the isolates into sensitive and tolerant. A high phenotype-genotype concordance (95%) regarding tolerance to QACs was obtained by screening the genomes for the presence of QAC tolerance-associated genes bcrABC, emrE, emrC, and qacH. Based on this high concordance, we assessed the QAC genes’ dissemination among publicly available L. monocytogenes genomes (n = 39,196). Overall, QAC genes were found in 23% and 28% of the L. monocytogenes collection in this study and in the global data set, respectively. bcrABC and qacH were the most prevalent genes, with bcrABC being the most detected QAC gene in the USA, while qacH dominated in Europe. No significant differences (P > 0.05) in the PAA tolerance were detected among isolates belonging to different lineages, serogroups, clonal complexes, or isolation sources, highlighting limited variation in the L. monocytogenes sensitivity to this disinfectant. The present work represents the largest testing of L. monocytogenes sensitivity to important food industry disinfectants at the phenotypic and genomic level, revealing diversity in the tolerance to QACs while all isolates showed similar sensitivity to PAA.
000152081 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttp://creativecommons.org/licenses/by/3.0/es/
000152081 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000152081 700__ $$aLaage Kragh, Martin
000152081 700__ $$aSzarvas, Judit
000152081 700__ $$aTosun, Elif Seyda
000152081 700__ $$aHolmud, Natacha Friis
000152081 700__ $$aGmeiner, Alexander
000152081 700__ $$aAmar, Corinne
000152081 700__ $$aGuldimann, Claudia
000152081 700__ $$aHuynh, TuAnh N.
000152081 700__ $$aKarpíšková, Renáta
000152081 700__ $$0(orcid)0000-0001-6375-9784$$aRota, Carmen$$uUniversidad de Zaragoza
000152081 700__ $$0(orcid)0000-0001-6194-8346$$aGomez, Diego$$uUniversidad de Zaragoza
000152081 700__ $$aAboagye, Eurydice
000152081 700__ $$aEtter, Andrea
000152081 700__ $$aCentorame, Patrizia
000152081 700__ $$aTorresi, Marina
000152081 700__ $$aDe Angelis, Maria Elisabetta
000152081 700__ $$aPomilio, Francesco
000152081 700__ $$aOkholm, Anders Hauge
000152081 700__ $$aXiao, Yinghua
000152081 700__ $$aKleta, Sylvia
000152081 700__ $$aLüth, Stefanie
000152081 700__ $$aPietzka, Ariane
000152081 700__ $$aKovacevic, Jovana
000152081 700__ $$aPagotto, Franco
000152081 700__ $$aRychli, Kathrin
000152081 700__ $$aZdovc, Irena
000152081 700__ $$aPapic, Bojan
000152081 700__ $$aHeir, Even
000152081 700__ $$aLangsrud, Solveig
000152081 700__ $$aMøretrø, Trond
000152081 700__ $$aBrown, Phillip
000152081 700__ $$aKathariou, Sophia
000152081 700__ $$aStephan, Roger
000152081 700__ $$aTasara, Taurai
000152081 700__ $$aDalgaard, Paw
000152081 700__ $$aNjage, Patrick Murigu Kamau
000152081 700__ $$aFagerlund, Annette
000152081 700__ $$aAarestrup, Frank
000152081 700__ $$aTruelstrup Hansen, Lisbeth
000152081 700__ $$aLeekitcharoenphon, Pimlapas
000152081 7102_ $$12008$$2640$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Nutrición Bromatología
000152081 773__ $$tApplied and Environmental Microbiology$$x0099-2240
000152081 787__ $$tRaw sequencing data$$whttps://www.ebi.ac.uk/ena/browser/view/PRJEB56155
000152081 8564_ $$s6471389$$uhttps://zaguan.unizar.es/record/152081/files/texto_completo.pdf$$yVersión publicada
000152081 8564_ $$s2541265$$uhttps://zaguan.unizar.es/record/152081/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000152081 909CO $$ooai:zaguan.unizar.es:152081$$particulos$$pdriver
000152081 951__ $$a2025-03-26-13:54:42
000152081 980__ $$aARTICLE