000152081 001__ 152081 000152081 005__ 20251017144622.0 000152081 0247_ $$2doi$$a10.1128/aem.01829-24 000152081 0248_ $$2sideral$$a143394 000152081 037__ $$aART-2025-143394 000152081 041__ $$aeng 000152081 100__ $$aIvanova, Mirena 000152081 245__ $$aLarge-scale phenotypic and genomic analysis of Listeria monocytogenes reveals diversity in the sensitivity to quaternary ammonium compounds but not to peracetic acid 000152081 260__ $$c2025 000152081 5060_ $$aAccess copy available to the general public$$fUnrestricted 000152081 5203_ $$aListeria monocytogenes presents a significant concern for the food industry due to its ability to persist in the food processing environment. One of the factors contributing to its persistence is decreased sensitivity to disinfectants. Our objective was to assess the diversity of L. monocytogenes sensitivity to food industry disinfectants by testing the response of 1,671 L. monocytogenes isolates to quaternary ammonium compounds (QACs) and 414 isolates to peracetic acid (PAA) using broth microdilution and growth curve analysis assays, respectively, and to categorize the isolates into sensitive and tolerant. A high phenotype-genotype concordance (95%) regarding tolerance to QACs was obtained by screening the genomes for the presence of QAC tolerance-associated genes bcrABC, emrE, emrC, and qacH. Based on this high concordance, we assessed the QAC genes’ dissemination among publicly available L. monocytogenes genomes (n = 39,196). Overall, QAC genes were found in 23% and 28% of the L. monocytogenes collection in this study and in the global data set, respectively. bcrABC and qacH were the most prevalent genes, with bcrABC being the most detected QAC gene in the USA, while qacH dominated in Europe. No significant differences (P > 0.05) in the PAA tolerance were detected among isolates belonging to different lineages, serogroups, clonal complexes, or isolation sources, highlighting limited variation in the L. monocytogenes sensitivity to this disinfectant. The present work represents the largest testing of L. monocytogenes sensitivity to important food industry disinfectants at the phenotypic and genomic level, revealing diversity in the tolerance to QACs while all isolates showed similar sensitivity to PAA. 000152081 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es 000152081 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion 000152081 700__ $$aLaage Kragh, Martin 000152081 700__ $$aSzarvas, Judit 000152081 700__ $$aTosun, Elif Seyda 000152081 700__ $$aHolmud, Natacha Friis 000152081 700__ $$aGmeiner, Alexander 000152081 700__ $$aAmar, Corinne 000152081 700__ $$aGuldimann, Claudia 000152081 700__ $$aHuynh, TuAnh N. 000152081 700__ $$aKarpíšková, Renáta 000152081 700__ $$0(orcid)0000-0001-6375-9784$$aRota, Carmen$$uUniversidad de Zaragoza 000152081 700__ $$0(orcid)0000-0001-6194-8346$$aGomez, Diego$$uUniversidad de Zaragoza 000152081 700__ $$aAboagye, Eurydice 000152081 700__ $$aEtter, Andrea 000152081 700__ $$aCentorame, Patrizia 000152081 700__ $$aTorresi, Marina 000152081 700__ $$aDe Angelis, Maria Elisabetta 000152081 700__ $$aPomilio, Francesco 000152081 700__ $$aOkholm, Anders Hauge 000152081 700__ $$aXiao, Yinghua 000152081 700__ $$aKleta, Sylvia 000152081 700__ $$aLüth, Stefanie 000152081 700__ $$aPietzka, Ariane 000152081 700__ $$aKovacevic, Jovana 000152081 700__ $$aPagotto, Franco 000152081 700__ $$aRychli, Kathrin 000152081 700__ $$aZdovc, Irena 000152081 700__ $$aPapic, Bojan 000152081 700__ $$aHeir, Even 000152081 700__ $$aLangsrud, Solveig 000152081 700__ $$aMøretrø, Trond 000152081 700__ $$aBrown, Phillip 000152081 700__ $$aKathariou, Sophia 000152081 700__ $$aStephan, Roger 000152081 700__ $$aTasara, Taurai 000152081 700__ $$aDalgaard, Paw 000152081 700__ $$aNjage, Patrick Murigu Kamau 000152081 700__ $$aFagerlund, Annette 000152081 700__ $$aAarestrup, Frank 000152081 700__ $$aTruelstrup Hansen, Lisbeth 000152081 700__ $$aLeekitcharoenphon, Pimlapas 000152081 7102_ $$12008$$2640$$aUniversidad de Zaragoza$$bDpto. Produc.Animal Cienc.Ali.$$cÁrea Nutrición Bromatología 000152081 773__ $$tApplied and Environmental Microbiology$$x0099-2240 000152081 787__ $$tRaw sequencing data$$whttps://www.ebi.ac.uk/ena/browser/view/PRJEB56155 000152081 8564_ $$s6471389$$uhttps://zaguan.unizar.es/record/152081/files/texto_completo.pdf$$yVersión publicada 000152081 8564_ $$s2541265$$uhttps://zaguan.unizar.es/record/152081/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada 000152081 909CO $$ooai:zaguan.unizar.es:152081$$particulos$$pdriver 000152081 951__ $$a2025-10-17-14:22:16 000152081 980__ $$aARTICLE