Novel intracellular antibiotic delivery system against Staphylococcus aureus: Cloxacillin-loaded poly(d, l-lactide-co-glycolide) acid nanoparticles

Lacoma, Alicia ; Usón, Laura (Universidad de Zaragoza) ; Mendoza, Gracia (Universidad de Zaragoza) ; Sebastián, Víctor (Universidad de Zaragoza) ; Garcia-Garcia, Esther ; Muriel-Moreno, Beatriz ; Domínguez, José ; Arruebo, Manuel (Universidad de Zaragoza) ; Prat, Cristina

Resumen: Aim: First, to compare in vitro minimum inhibitory concentrations (MIC) of free cloxacillin and cloxacillin-containing nanoparticles (NP) against methicillin-susceptible (MSSA) and resistant Staphylococcus aureus (MRSA) and second, to assess NP antimicrobial activity against intracellular S. aureus. Methods: Poly(d, l-lactide-co-glycolide) acid (PLGA)-NP were loaded with cloxacillin and physico-chemically characterized. MICs were determined for reference strains Newman-(MSSA) and USA300-(MRSA). Murine alveolar macrophages were infected, and bacterial intracellular survival was assessed after incubating with free-cloxacillin or PLGA-cloxacillin-NP. Results & conclusion: For both isolates, MICs for antibiotic-loaded-NP were lower than those obtained with free cloxacillin, indicating that the drug encapsulation improves antimicrobial activity. A sustained antibiotic release was demonstrated when using the PLGA-cloxacillin-NP. When considering the lowest concentrations, the use of drug-loaded NP enabled a higher reduction of intracellular bacterial load.

Idioma: Inglés

DOI: 10.2217/nnm-2019-0371

Año: 2020

Publicado en: Nanomedicine 15, 12 (2020), 1189-1203
ISSN: 1743-5889

Factor impacto JCR: 5.307 (2020)

Categ. JCR: BIOTECHNOLOGY & APPLIED MICROBIOLOGY rank: 30 / 158 = 0.19 (2020) - Q1 - T1
Categ. JCR: NANOSCIENCE & NANOTECHNOLOGY rank: 46 / 106 = 0.434 (2020) - Q2 - T2

Factor impacto SCIMAGO: 0.946 - Bioengineering (Q1) - Biomedical Engineering (Q1) - Nanoscience and Nanotechnology (Q1) - Materials Science (miscellaneous) (Q1) - Medicine (miscellaneous) (Q1) - Development (Q1)

Financiación: info:eu-repo/grantAgreement/EUR/ERC-2013-CoG-614715-NANOHEDONISM
Financiación: info:eu-repo/grantAgreement/ES/ISCIII/FEDER/PI17-01139
Financiación: info:eu-repo/grantAgreement/ES/MICINN/CTQ2017-84473-R

Tipo y forma: Article (Published version)

Área (Departamento): Área Ingeniería Química (Dpto. Ing.Quím.Tecnol.Med.Amb.)