000156674 001__ 156674
000156674 005__ 20251017144613.0
000156674 0247_ $$2doi$$a10.1002/adfm.202420106
000156674 0248_ $$2sideral$$a143959
000156674 037__ $$aART-2025-143959
000156674 041__ $$aeng
000156674 100__ $$aIglesias-García, Olalla
000156674 245__ $$aBiologically-Inspired Melt Electrowriting for the Generationof Highly Biomimetic Functional Myocardium
000156674 260__ $$c2025
000156674 5060_ $$aAccess copy available to the general public$$fUnrestricted
000156674 5203_ $$aIn the heart, the specific 3D structure of myocardial layers produces an efficient ejection of blood. When myocardial infarction strikes, this architecture is disrupted, adding a disarranged contraction to the decreased availability of pumping units (cardiomyocytes, CMs). In this work, the alignment of cardiac fibers in a large animal model (pig) is characterized and employ melt electrowriting (MEW) to fabricate a bio-inspired scaffold with diamond-shaped pores. Using human-induced pluripotent stem cell-derived CMs and cardiac fibroblasts, human cardiac tissues with a biomimetic in-plane contraction are generated. MEW-diamond tissues beat macroscopically for over 1 month, with significantly faster kinetics, increased force, and higher conduction velocity than those based on square or rectangular pores. The diamond design induces a specific hiPSC-CM alignment resulting in the observed in-plane contraction. Transcriptomic analysis using bulk RNA-seq reveals diamond-MEW tissues present features of maturation as compared to traditional 2D cultures. Finally, the bio-inspired cardiac tissues are employed to treat an infarction model in athymic rats, showing a significant benefit on systolic function and remodeling, tied to the presence of large grafts of human cells remuscularizing the ventricular wall. All in all, it is demonstrated that the new design generates superior human cardiac tissues with therapeutic capacity.
000156674 536__ $$9info:eu-repo/grantAgreement/ES/DGA/T39-23R$$9info:eu-repo/grantAgreement/EC/H2020/874827/EU/Computational biomechanics and bioengineering 3D printing to develop a personalized regenerative biological ventricular assist device to provide lasting functional support to damaged hearts/BRAV3$$9This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No H2020 874827-BRAV3$$9info:eu-repo/grantAgreement/ES/ISCIII/CD22-00027$$9info:eu-repo/grantAgreement/ES/MCIN/INVESTTRA_PID2022-142807OA-I00$$9info:eu-repo/grantAgreement/ES/MCIN/PLEC2021-008127$$9info:eu-repo/grantAgreement/ES/MCIN/VOLVAD_PID2022-142562OB-I00$$9info:eu-repo/grantAgreement/ES/MICINN/PID2022-140556OB-I00$$9info:eu-repo/grantAgreement/EUR/MICINN/TED2021-130459B-I00
000156674 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000156674 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000156674 700__ $$aFlandes-Iparraguirre, María
000156674 700__ $$aMontero-Calle, Pilar
000156674 700__ $$aRosales, Ricardo M.
000156674 700__ $$aUllate-Agote, Asier
000156674 700__ $$aSánchez-Bueno, Andrea
000156674 700__ $$aLarequi, Eduardo
000156674 700__ $$aAnaut-Lusar, Ilazki
000156674 700__ $$0(orcid)0000-0001-8946-4829$$aLaita, Nicolás
000156674 700__ $$0(orcid)0000-0001-5348-924X$$aOliván-Viguera, Aida
000156674 700__ $$aIglesias, Elena
000156674 700__ $$aAbizanda, Gloria
000156674 700__ $$aSan Martín-Úriz, Patxi
000156674 700__ $$aAguirre-Ruiz, Paula
000156674 700__ $$aAranguren, Xabier L.
000156674 700__ $$aGarcía de Yébenes, Manuel
000156674 700__ $$aGavira, Juan José
000156674 700__ $$aMartínez, Miguel Ángel
000156674 700__ $$0(orcid)0000-0002-0664-5024$$aPeña, Estefanía$$uUniversidad de Zaragoza
000156674 700__ $$0(orcid)0000-0001-8741-6452$$aDoblaré, Manuel$$uUniversidad de Zaragoza
000156674 700__ $$ade-Juan-Pardo, Elena M.
000156674 700__ $$0(orcid)0000-0002-1960-407X$$aPueyo, Esther$$uUniversidad de Zaragoza
000156674 700__ $$aProsper, Felipe
000156674 700__ $$aMazo Vega, Manuel M.
000156674 7102_ $$15008$$2800$$aUniversidad de Zaragoza$$bDpto. Ingeniería Electrón.Com.$$cÁrea Teoría Señal y Comunicac.
000156674 7102_ $$15004$$2605$$aUniversidad de Zaragoza$$bDpto. Ingeniería Mecánica$$cÁrea Mec.Med.Cont. y Teor.Est.
000156674 773__ $$g(2025), 2420106 [20 pp.]$$pAdv. funct. mater.$$tAdvanced Functional Materials$$x1616-301X
000156674 8564_ $$s4439329$$uhttps://zaguan.unizar.es/record/156674/files/texto_completo.pdf$$yVersión publicada
000156674 8564_ $$s2706181$$uhttps://zaguan.unizar.es/record/156674/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000156674 909CO $$ooai:zaguan.unizar.es:156674$$particulos$$pdriver
000156674 951__ $$a2025-10-17-14:18:16
000156674 980__ $$aARTICLE