Enhancing Candida auris diagnosis: a comprehensive evaluation of VIASURE Candida auris real-time PCR detection kit for rapid diagnostic responses

Dehesa-García, Blanca ; Alonso, Henar ; Peris, María Paz (Universidad de Zaragoza) ; Fortuño, Blanca ; Abad, Pilar ; Rezusta, Antonio ; Milagro, Ana

Resumen: Candida auris, a yeast that is highly resistant to antifungal treatments, represents a significant healthcare concern due to its rapid spread through surface contamination and patient colonization. A recent outbreak at the Miguel Servet University Hospital in Zaragoza, Spain, highlighted the urgent need for rapid diagnostic responses. The current 4-day duration for microbiological culture results hinders timely patient isolation and transmission prevention. This study aims to evaluate the VIASURE Candida auris Real-Time PCR Detection Kit (Certest Biotec) for diagnostic performance and analytical specificity compared to the standard methodology based on culture and MALDI-TOF MS characterization. From August 2023 to December 2023, the Microbiology Service of the Miguel Servet University Hospital processed 816 diagnostic samples. Accuracy testing resulted in adequate clinical validation values, as presented here: sensitivity, 0.98 (95% confidence interval [CI], 0.90 to 1); specificity, 1 (95% CI, 0.99 to 1); positive predictive value, 0.98 (95% CI, 0.90 to 1); and negative predictive value, 1 (95% CI, 0.99 to 1). The implementation of this molecular biology test in the laboratory promises to improve diagnostic efficiency by allowing rapid patient isolation, initiation of appropriate treatment, and effective isolation measures.

Idioma: Inglés

DOI: 10.1128/spectrum.03111-24

Año: 2025

Publicado en: Microbiology Spectrum (2025), e03111-24 [10 pp.]
ISSN: 2165-0497

Tipo y forma: Article (Published version)

Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)