000163826 001__ 163826
000163826 005__ 20251107115328.0
000163826 0247_ $$2doi$$a10.1108/RPJ-11-2024-0491
000163826 0248_ $$2sideral$$a145985
000163826 037__ $$aART-2025-145985
000163826 041__ $$aeng
000163826 100__ $$aTeixeira, Ana Margarida
000163826 245__ $$aFabrication and characterization of an agarose and GelMA hybrid scaffold for adipose tissue regeneration
000163826 260__ $$c2025
000163826 5060_ $$aAccess copy available to the general public$$fUnrestricted
000163826 5203_ $$aPurpose
This study aims to overcome the drawbacks of current clinical practices in breast reconstruction. Tissue engineering is currently the most promising field to create improved alternatives and new strategies. Biomaterials that mimic the physico-chemical and biological properties of the native extracellular matrix (ECM), e.g. hydrogels, have been characterized and seeded with cells, with the purpose of promoting the regeneration of tissues. In this study, a new hydrogel blend was produced and characterized for adipose tissue regeneration purposes.
Design/methodology/approach
Blends of agarose and gelatin-methacryloyl (GelMA) were produced in different weight percentage ratios (100:0, 75:25 and 50:50 of agarose:GelMA). Indirect 3D printed scaffolds and 2D discs were fabricated and characterized in terms of physico-chemical and biological properties in the presence of adipose tissue-derived stem cells.
Findings
Both scaffolds and discs presented a good gel fraction (>90% for most of the samples) and a swelling degree >17% for all samples. In terms of stiffness, the results varied from 2.86 kPa to 25.11 kPa depending on the testing conditions (i.e. testing temperature) and sample chemical composition. Rheology analysis showed that the storage modulus was higher than the loss modulus, indicating a more elastic behavior. Moreover, the scaffolds with GelMA could support cell adhesion and proliferation, with a viability higher than the control (i.e. tissue culture plastic, TCP) by day 14 (118% and 128% for the ratios 75:25 and 50:50, respectively), showing that 50:50 is the optimum ratio composition.
Originality/value
The blend of agarose GelMA presented in this study offers a new and promising approach toward adipose tissue regeneration.
000163826 540__ $$9info:eu-repo/semantics/openAccess$$aby-nc$$uhttps://creativecommons.org/licenses/by-nc/4.0/deed.es
000163826 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/acceptedVersion
000163826 700__ $$aVan Damme, Lana
000163826 700__ $$aVan Vlierberghe, Sandra
000163826 700__ $$0(orcid)0000-0001-9732-4498$$ade Sousa Martins, Pedro Alexandre Lopes
000163826 773__ $$g31, 9 (2025), 1933-1947$$pRapid prototyping j.$$tRAPID PROTOTYPING JOURNAL$$x1355-2546
000163826 8564_ $$s4127701$$uhttps://zaguan.unizar.es/record/163826/files/texto_completo.pdf$$yPostprint
000163826 8564_ $$s2418456$$uhttps://zaguan.unizar.es/record/163826/files/texto_completo.jpg?subformat=icon$$xicon$$yPostprint
000163826 909CO $$ooai:zaguan.unizar.es:163826$$particulos$$pdriver
000163826 951__ $$a2025-11-07-10:25:28
000163826 980__ $$aARTICLE