Scope and Synthetic Applications of the Aryl-Alcohol Oxidase from <i>Streptomyces hiroshimensis</i> ( <i>Sh</i> AAO)

Ascaso-Alegre, Christian ; Cinca-Fernando, Paula (Universidad de Zaragoza) ; Roberts, Tom L. ; López-Fernández, Pablo ; Pérez Herrera, Raquel ; Cosgrove, Sebastian C. ; Ferreira, Patricia (Universidad de Zaragoza) ; Mangas-Sánchez, Juan

Resumen: The sustainable production of carbonyl compounds is of growing interest due to their broad academic and industrial interest. Herein, we explored the aryl-alcohol oxidase from Streptomyces hiroshimensis (ShAAO), mapping its activity across diverse alcohols, revealing a distinct profile from other natural AAOs. Integrated into tandem organocatalytic processes, ShAAO enabled the synthesis of various chiral compounds, including an intermediate for paroxetine. Gram-scale reactions delivered high yields and turnover numbers exceeding 65000. Immobilization further enhanced stability, highlighting ShAAO as an efficient and industrially relevant biocatalyst.

Idioma: Inglés

DOI: 10.1021/acs.orglett.5c03814

Año: 2025

Publicado en: ORGANIC LETTERS 27, 43 (2025), 12086-12091
ISSN: 1523-7060

Financiación: info:eu-repo/grantAgreement/ES/AEI/PID2023-147471NB-I00
Financiación: info:eu-repo/grantAgreement/ES/DGA/E07-23R
Financiación: info:eu-repo/grantAgreement/ES/MICINN/PID2022-136369NB-I00
Financiación: info:eu-repo/grantAgreement/EUR/MICINN/TED2021-130803B-I00

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)