Effect of a Reduced-Protein Diet Supplemented with Essential Amino Acids on the Muscle Proteome of Female and Entire Male Finishing Pigs

de Almeida, André M. ; Osório, Hugo ; Latorre, María Ángeles (Universidad de Zaragoza) ; Álvarez-Rodríguez, Javier (Universidad de Zaragoza)

Resumen: Crude protein (CP) decrease coupled to amino acid (AAs) supplementation is interesting for swine nutrition. A proteomics approach unraveled physiological events underlying differences between sexes fed two diets: control and low CP in fast-growing Duroc X (Landrace X Large white) finishing pigs. Sixty animals per sex were distributed in pens (five replicate pens/group), fed ad libitum with two isoenergetic diets (145 vs. 125 g CP/kg with AA supplementation; control and low-CP) for 42 days. Semimembranosus muscle was sampled. Five samples per group were used for Label Free proteomics. Four comparisons were considered: Control Males vs. Control Females; Control Females vs. Low CP females; Control males vs. Low CP males, and Low CP males vs. Low CP females, identifying, respectively, 26, 19, 12, and 11 DAPs (Differentially Accumulated Proteins). Control males had higher abundance of proteins related to cell differentiation and growth compared to females, highlighting continual muscle accretion in the former and lipogenesis onset in the latter. Control females and males had increased DAPs related to tissue growth and differentiation compared to Low CP animals that had increased lipid accretion. Both sexes reacted similarly to the two diets. Low CP diet led to advanced lipogenesis and decreased muscle accretion pathways.

Idioma: Inglés

DOI: 10.3390/ani15223325

Año: 2025

Publicado en: Animals 15, 22 (2025), 3325 [21]
ISSN: 2076-2615

Tipo y forma: Artículo (Versión definitiva)

Área (Departamento): Área Producción Animal (Dpto. Produc.Animal Cienc.Ali.)