On-chip EPR spectrometry of metalloproteins using superconducting lumped element resonators
Marcuello, Carlos ; Rodriguez, David ; Pallarés, María Carmen ; Granados, Daniel ; Roubeau, Olivier ; Luis, Fernando ; Gomez, Alicia ; Lostao, Anabel
Resumen: We report electron paramagnetic resonance experiments performed on myoglobin hemeproteins using a chip hosting 6 superconducting lumped element resonators with resonance frequencies between 1.94 and 2.11 GHz. Successive layers of myoglobin were deposited onto the inductors of four of them using dip-pen nanolithography, a technique based on atomic force microscopy. A combination of atomic force and confocal microscopies estimated the number of protein molecules in each deposit, which ranges from 8.6 × 10^11 (one dip-pen layer) to 3.33 × 10^12 (four dip-pen layers). Two reference bulk samples were pipetted from the same solution onto the remaining two resonators. The microwave transmission of the device, measured at 11 mK, shows evidence of the coupling of protein spins to the photon excitations of all resonators. In particular, the resonance broadening measured as a function of magnetic field provides the spin resonance absorption spectrum. The analysis suggests that proteins tend to self-orient on the chip. It also allows estimating the single spin to single photon coupling strength, which is around 9 Hz. This high coupling value suggests that dip-pen nanolithography gives rise to a close to optimum interface between the molecules and the chip surface. The developed methodology combines an increase in sensitivity of at least three orders of magnitude with the ability to characterize multiple samples in a single experiment, opening the door to a highly sensitive multi-analyte detection technology.
Idioma: Inglés
DOI: 10.1039/D5NR03119B
Año: 2025
Publicado en: Nanoscale (2025), [9 pp.]
ISSN: 2040-3364
Financiación: info:eu-repo/grantAgreement/ES/AEI/AEI PID2022-137779OB-C41
Financiación: info:eu-repo/grantAgreement/ES/AEI/AEI PID2022-140923NB-C21
Financiación: info:eu-repo/grantAgreement/ES/DGA/E09-23R
Financiación: info:eu-repo/grantAgreement/ES/DGA/E31-23R
Financiación: info:eu-repo/grantAgreement/ES/MICINN/CEX2020-001039-S
Financiación: info:eu-repo/grantAgreement/EUR/MICINN/TED2021-131447B-C21
Financiación: info:eu-repo/grantAgreement/EUR/MICINN/TED2021-131447B-C22
Financiación: info:eu-repo/grantAgreement/ES/MICIU/CEX2023-001286-S
Financiación: info:eu-repo/grantAgreement/ES/MINECO/PID2020-118329RB-I00
Tipo y forma: Article (Published version)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes.
Exportado de SIDERAL (2026-01-07-18:53:33)
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Idioma: Inglés
DOI: 10.1039/D5NR03119B
Año: 2025
Publicado en: Nanoscale (2025), [9 pp.]
ISSN: 2040-3364
Financiación: info:eu-repo/grantAgreement/ES/AEI/AEI PID2022-137779OB-C41
Financiación: info:eu-repo/grantAgreement/ES/AEI/AEI PID2022-140923NB-C21
Financiación: info:eu-repo/grantAgreement/ES/DGA/E09-23R
Financiación: info:eu-repo/grantAgreement/ES/DGA/E31-23R
Financiación: info:eu-repo/grantAgreement/ES/MICINN/CEX2020-001039-S
Financiación: info:eu-repo/grantAgreement/EUR/MICINN/TED2021-131447B-C21
Financiación: info:eu-repo/grantAgreement/EUR/MICINN/TED2021-131447B-C22
Financiación: info:eu-repo/grantAgreement/ES/MICIU/CEX2023-001286-S
Financiación: info:eu-repo/grantAgreement/ES/MINECO/PID2020-118329RB-I00
Tipo y forma: Article (Published version)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes. Exportado de SIDERAL (2026-01-07-18:53:33)
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Record created 2026-01-07, last modified 2026-01-07