Advances in genotyping microsatellite markers through sequencing and consequences of scoring methods for <i>Ceratonia siliqua</i> (Leguminosae)
Viruel, Juan ; Haguenauer, Anne ; Juin, Marianick ; Mirleau, Fatma ; Bouteiller, Delphine ; Boudagher-Kharrat, Magda ; Ouahmane, Lahcen ; La Malfa, Stefano ; Médail, Frédéric ; Sanguin, Hervé ; Nieto Feliner, Gonzalo ; Baumel, Alex
Resumen: Premise of the Study
Simple sequence repeat (SSR) or microsatellite markers have been used in a broad range of studies mostly scoring alleles on the basis of amplicon size as a proxy for the number of repeat units of an SSR motif. However, additional sources of variation within the SSR or in the flanking regions have largely remained undetected.
Methods
In this study, we implemented a next-generation sequencing–based genotyping approach in a newly characterized set of 18 nuclear SSR markers for the carob tree, Ceratonia siliqua. Our aim was to evaluate the effect of three different methods of scoring molecular variation present within microsatellite markers on the genetic diversity and structure results.
Results
The analysis of the sequences of 77 multilocus genotypes from four populations revealed SSR variation and additional sources of polymorphism in 87% of the loci analyzed (42 single-nucleotide polymorphisms and five insertion/deletion polymorphisms), as well as divergent paralog copies in two loci. Ignoring sequence variation under standard amplicon size genotyping resulted in incorrect identification of 69% of the alleles, with important effects on the genetic diversity and structure estimates.
Discussion
Next-generation sequencing allows the detection and scoring of SSRs, single-nucleotide polymorphisms, and insertion/deletion polymorphisms to increase the resolution of population genetic studies.
Idioma: Inglés
DOI: 10.1002/aps3.1201
Año: 2018
Publicado en: Applications in plant sciences 6, 12 (2018), e1201 [10 pp.]
ISSN: 2168-0450
Factor impacto JCR: 1.232 (2018)
Categ. JCR: PLANT SCIENCES rank: 137 / 226 = 0.606 (2018) - Q3 - T2
Factor impacto SCIMAGO: 0.607 - Plant Science (Q2) - Ecology, Evolution, Behavior and Systematics (Q2)
Tipo y forma: Artículo (Versión definitiva)
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Exportado de SIDERAL (2026-01-12-11:08:43)
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Simple sequence repeat (SSR) or microsatellite markers have been used in a broad range of studies mostly scoring alleles on the basis of amplicon size as a proxy for the number of repeat units of an SSR motif. However, additional sources of variation within the SSR or in the flanking regions have largely remained undetected.
Methods
In this study, we implemented a next-generation sequencing–based genotyping approach in a newly characterized set of 18 nuclear SSR markers for the carob tree, Ceratonia siliqua. Our aim was to evaluate the effect of three different methods of scoring molecular variation present within microsatellite markers on the genetic diversity and structure results.
Results
The analysis of the sequences of 77 multilocus genotypes from four populations revealed SSR variation and additional sources of polymorphism in 87% of the loci analyzed (42 single-nucleotide polymorphisms and five insertion/deletion polymorphisms), as well as divergent paralog copies in two loci. Ignoring sequence variation under standard amplicon size genotyping resulted in incorrect identification of 69% of the alleles, with important effects on the genetic diversity and structure estimates.
Discussion
Next-generation sequencing allows the detection and scoring of SSRs, single-nucleotide polymorphisms, and insertion/deletion polymorphisms to increase the resolution of population genetic studies.
Idioma: Inglés
DOI: 10.1002/aps3.1201
Año: 2018
Publicado en: Applications in plant sciences 6, 12 (2018), e1201 [10 pp.]
ISSN: 2168-0450
Factor impacto JCR: 1.232 (2018)
Categ. JCR: PLANT SCIENCES rank: 137 / 226 = 0.606 (2018) - Q3 - T2
Factor impacto SCIMAGO: 0.607 - Plant Science (Q2) - Ecology, Evolution, Behavior and Systematics (Q2)
Tipo y forma: Artículo (Versión definitiva)
Debe reconocer adecuadamente la autoría, proporcionar un enlace a la licencia e indicar si se han realizado cambios. Puede hacerlo de cualquier manera razonable, pero no de una manera que sugiera que tiene el apoyo del licenciador o lo recibe por el uso que hace. No puede utilizar el material para una finalidad comercial. Si remezcla, transforma o crea a partir del material, no puede difundir el material modificado.Exportado de SIDERAL (2026-01-12-11:08:43)
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