000165692 001__ 165692
000165692 005__ 20260113234334.0
000165692 0247_ $$2doi$$a10.1371/journal.pone.0164883
000165692 0248_ $$2sideral$$a129712
000165692 037__ $$aART-2016-129712
000165692 041__ $$aeng
000165692 100__ $$aPuigvehí, Marc
000165692 245__ $$aDiagnostic Accuracy of the Enhanced Liver Fibrosis (ELF\u00ae) Score Using HCV-Infected Serum Samples Cryopreserved for up to 25 Years
000165692 260__ $$c2016
000165692 5060_ $$aAccess copy available to the general public$$fUnrestricted
000165692 5203_ $$aIntroduction & Aims
Cryopreservation of serum samples is a standard procedure for biomedical research in tertiary centers. However, studies evaluating the long-term biological stability of direct liver fibrosis markers using cryopreserved samples are scarce.
Methods
We compared the stability of hyaluronic acid (HA), tissue inhibitor of metalloproteinases (TIMP-1) and amino-terminal propeptide of type III procollagen (PIIINP) in 225 frozen serum samples of HCV-infected patients with a paired liver biopsy for up to 25 years (1990–2014). Moreover, we assessed the diagnostic accuracy (AUROC) of the Enhanced Liver Fibrosis (ELF®) score to identify significant fibrosis (F2-4) and its predictive capacity to identify clinical events during follow-up.
Results
Seventy-six patients (39,8%) had mild fibrosis (F0-1) and 115 (60,2%) significant fibrosis (F2-4). HA, PIIINP and TIMP-1 values remained stable during the period from 1995 to 2014 while those of 1990–94 were slightly higher. We did not find significant differences in the median ELF® values during the 20-year period from 1995–2014 in patients with mild (from 8,4 to 8,7) and significant fibrosis (from 9,9 to 10,9) (p = ns between periods and fibrosis stages). The AUROCs of ELF® to identify significant fibrosis were high in all the periods (from 0,85 to 0,91). The ELF® score showed a good predictive capability to identify clinical events during follow-up.
Conclusions
The biological stability of direct serum markers (HA, PIIINP and TIMP-1) using HCV-infected samples cryopreserved for 20 years is good. Therefore, the diagnostic accuracy of the ELF® score to identify significant fibrosis and clinical events during follow-up is very high.
000165692 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000165692 590__ $$a2.806$$b2016
000165692 591__ $$aMULTIDISCIPLINARY SCIENCES$$b15 / 63 = 0.238$$c2016$$dQ1$$eT1
000165692 592__ $$a1.236$$b2016
000165692 593__ $$aAgricultural and Biological Sciences (miscellaneous)$$c2016$$dQ1
000165692 593__ $$aMedicine (miscellaneous)$$c2016$$dQ1
000165692 593__ $$aBiochemistry, Genetics and Molecular Biology (miscellaneous)$$c2016$$dQ1
000165692 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000165692 700__ $$aHernández, Juanjo
000165692 700__ $$aBroquetas, Teresa
000165692 700__ $$aColl, Susanna
000165692 700__ $$aGarcia-Retortillo, Montserrat
000165692 700__ $$aCañete, Nuria
000165692 700__ $$aGiménez, Maria Dolors
000165692 700__ $$0(orcid)0000-0003-2078-8205$$aGarcia, Mar
000165692 700__ $$aBory, Felipe
000165692 700__ $$aSalvadó, Margarita
000165692 700__ $$aSola, Ricard
000165692 700__ $$aCarrión, José A.
000165692 700__ $$aStrnad, Pavel
000165692 773__ $$g11, 12 (2016), e0164883$$pPLoS One$$tPLoS ONE$$x1932-6203
000165692 8564_ $$s1142406$$uhttps://zaguan.unizar.es/record/165692/files/texto_completo.pdf$$yVersión publicada
000165692 8564_ $$s1999164$$uhttps://zaguan.unizar.es/record/165692/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000165692 909CO $$ooai:zaguan.unizar.es:165692$$particulos$$pdriver
000165692 951__ $$a2026-01-13-22:06:04
000165692 980__ $$aARTICLE