Reproducibility and sources of variation of urinary biomarkers of food intake of fruits, vegetables, and chocolate in european children and adolescents

Goerdten, Jantje ; Rattner, Jodi ; Merdas, Mira ; Achaintre, David ; Yuan, Li ; Russo, Paola ; Veidebaum, Toomas ; Molnár, Dénes ; Lissner, Lauren ; De Henauw, Stefaan ; Moreno, Luis A (Universidad de Zaragoza) ; Aleksandrova, Krasimira ; Foraita, Ronja ; Nöthlings, Ute ; Keski-Rahkonen, Pekka ; Floegel, Anna

Resumen: Background: Biomarkers of food intake may improve dietary assessment. Thereby, a key concern is their reproducibility over time. In epidemiological studies, it is important to accurately estimate habitual food intake and consequent disease risk associations. Objectives: We aimed to assess the reproducibility of 12 urinary metabolites linked to food intake and to investigate potential sources of
their variation. Methods: The analyses are based on previously identified urinary metabolites associated with dietary intake of fruits, vegetables, and chocolate in the large-scale European Identification and Prevention of Dietary- and Lifestyle-Induced Health Effects in Children and Infants/I.Family study. Metabolites were measured in 1788 urine samples from 599 children at study baseline (2007/2008, n = 597), at the first follow-up (2009/2010, n = 596), and at the third follow-up (2013/2014, n = 595) using high-resolution liquid chromatography–MS. Unadjusted and adjusted intraclass correlation coefficients (ICCs) were calculated for 2- and 4-y intervals. To identify sources of biomarker variability, various factors, including dietary intake, were analyzed. The amount of variance explained by each factor was quantified using the partial coefficient of determination (R2). Results: The median ICCs were 0.27 (range: 0.11–0.54) and 0.28 (range: 0.15–0.51) over 2- and 4-y intervals, respectively. Individual factors explained a median of 17% (range: 9.8–42.4) of the variance for the 2-y interval and 14.6% (range: 8.3–43.8) for the 4-y interval. Country of residence explained the largest proportion of variance (median: 5% for the 2-y interval, 4.5% for the 4-y interval). Dietary intake explained only a variation of 0.7% (0.0–1.5) and 0.6% (0.0–1.1) for the 2- and 4-y interval, respectively.

Idioma: Inglés

DOI: 10.1016/j.tjnut.2025.10.039

Año: 2025

Publicado en: The Journal of nutrition 156, 1 (2025), 101226 [10 pp.]
ISSN: 0022-3166

Financiación: info:eu-repo/grantAgreement/EUR/FP6/FOOD-016181
Financiación: info:eu-repo/grantAgreement/EC/FP7/266044/EU/Determinants of eating behaviour in European children, adolescents and their parents/I.FAMILY

Tipo y forma: Article (Published version)

Área (Departamento): Área Enfermería (Dpto. Fisiatría y Enfermería)