Cyanobacterial Electron Carrier Proteins as Electron Donors to CYP106A2 from Bacillus Megaterium ATCC 13368
Goñi, G ; Zoellner, A ; Lisurek, M ; Velazquez-Campoy, A (Universidad de Zaragoza) ; Pinto, S ; Gomez-Moreno, C (Universidad de Zaragoza) ; Hannemann, F ; Bernhardt, R ; Medina, M. (Universidad de Zaragoza)
Resumen: The CYP450 from Bacillus megaterium (BmCYP106A2) catalyzes the 15βhydroxylation of several steroids and also synthesizes mono-hydroxylated 9α- and 11αOH-progesterone BmCYP106A2. The present study reports on the ability of BmCYP106A2 to be efficiently reduced by a truncated bovine adrenodoxin mutant, as well as by the photosynthetic flavodoxin and particularly ferredoxin electron carriers from the cyanobacterium Anabaena. These results open the possibility for the design of a hybrid system to provide reducing equivalents for the hydroxylation process. Additionally, they suggest that despite the interaction of BmCYP106A2 with these
proteins, particularly with flavodoxin, do not rely on a precise complementarity of the reacting molecules, rearrangements might be required and alternative binding modes might contribute to the observed ET reactions.
Idioma: Inglés
Año: 2009
Publicado en: Biochimica et Biophysica Acta - Proteins and Proteomics 1794, 11 (2009), 1635-1642
ISSN: 1570-9639
Factor impacto JCR: 2.48 (2009)
Categ. JCR: BIOPHYSICS rank: 41 / 74 = 0.554 (2009) - Q3 - T2
Categ. JCR: BIOCHEMISTRY & MOLECULAR BIOLOGY rank: 153 / 281 = 0.544 (2009) - Q3 - T2
Tipo y forma: Article (PostPrint)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes. If you remix, transform, or build upon the material, you may not distribute the modified material.
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proteins, particularly with flavodoxin, do not rely on a precise complementarity of the reacting molecules, rearrangements might be required and alternative binding modes might contribute to the observed ET reactions.
Idioma: Inglés
Año: 2009
Publicado en: Biochimica et Biophysica Acta - Proteins and Proteomics 1794, 11 (2009), 1635-1642
ISSN: 1570-9639
Factor impacto JCR: 2.48 (2009)
Categ. JCR: BIOPHYSICS rank: 41 / 74 = 0.554 (2009) - Q3 - T2
Categ. JCR: BIOCHEMISTRY & MOLECULAR BIOLOGY rank: 153 / 281 = 0.544 (2009) - Q3 - T2
Tipo y forma: Article (PostPrint)
Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)
You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not use the material for commercial purposes. If you remix, transform, or build upon the material, you may not distribute the modified material. Exportado de SIDERAL (2026-02-09-14:41:49)
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Record created 2026-02-09, last modified 2026-02-09