Quantification of angiogenic sprouting under different growth factors in a microfluidic platform

Del Amo, C. (Universidad de Zaragoza) ; Borau, C. (Universidad de Zaragoza) ; Gutiérrez, R. ; Asín, J. (Universidad de Zaragoza) ; García-Aznar, J.M. (Universidad de Zaragoza)

Resumen: Angiogenesis, as example of collective migration of endothelial cells (ECs), is the main dynamic process that culminates in sprout formation from existing vessels. After tissue injury, the vascularity is interrupted, triggering the regeneration process and the release of different growth factors (GFs). The main aim of this work is to quantify the effect of specific GFs during the initial stage of sprout formation, namely: VEGF, PDGF-BB, TGFß and BMP-2, all of them involved in regenerative processes. For this purpose, we designed a novel algorithm implemented in Matlab to quantify the advance of the EC monolayer over time and the sprout structure in 3D. Our results show that VEGF is the main regulatory GF on angiogenesis processes, producing longer sprouts with higher frequency. However, the chemoattractant effect of VEGF depends on its concentration and its spatiotemporal location, having a critical impact on the sprout time evolution. PDGF-BB (namely as PDGF) has a global negative effect on both the number and length of sprouts. TGFß enhances sprout length at earlier times, although its effect gradually disappears over time. Finally, BMP-2 produces, overall, less number and shorter sprouts, but was the only GF with a positive evolution at longer times, producing fewer but longer sprouts.

Idioma: Inglés

DOI: 10.1016/j.jbiomech.2015.10.026

Año: 2016

Publicado en: Journal of Biomechanics 49, 8 (2016), 1340-1346
ISSN: 0021-9290

Factor impacto JCR: 2.664 (2016)

Categ. JCR: ENGINEERING, BIOMEDICAL rank: 25 / 77 = 0.325 (2016) - Q2 - T1
Categ. JCR: BIOPHYSICS rank: 32 / 73 = 0.438 (2016) - Q2 - T2

Factor impacto SCIMAGO: 1.242 - Biomedical Engineering (Q1) - Biophysics (Q1) - Sports Science (Q1) - Rehabilitation (Q1) - Orthopedics and Sports Medicine (Q1)

Financiación: info:eu-repo/grantAgreement/EUR/FP7/ERC2012-StG-306751
Financiación: info:eu-repo/grantAgreement/ES/MEC/FPU13-03194
Financiación: info:eu-repo/grantAgreement/ES/MINECO/DPI2012-38090-C03-01

Tipo y forma: Artículo (PostPrint)

Área (Departamento): Área Estadís. Investig. Opera. (Dpto. Métodos Estadísticos)
Área (Departamento): Área Mec.Med.Cont. y Teor.Est. (Dpto. Ingeniería Mecánica)