DNA Barcoding of Mosquitoes from the Pantanos de Centla Biosphere Reserve, Southeastern Mexico

Ortega-Morales, Aldo I. ; Hernández-Triana, Luis M. ; Chan-Chable, Rahuel J. ; Garza-Hernández, Javier A. ; González-Álvarez, Vicente H. ; Ruiz-Arrondo, Ignacio (Universidad de Zaragoza) ; Nikolova, Nadya I. ; MartÍnez-Arce, Arely ; Fooks, Anthony R. ; Rodríguez-Pérez, Mario A.

Resumen: Accurate identification of mosquito species is essential to support programs that involve the study of distribution and mosquito control. Numerous mosquito species are difficult to identify based only on morphological characteristics, due to the morphological similarities in different life stages and large numbers of some species that are members of morphologically similar species complexes. In the present study, the mosquitoes collected in the Pantanos de Centla Biosphere Reserve, southeastern Mexico, were evaluated using a combination of morphological and molecular approaches (mitochondrial cytochrome c oxidase subunit I [COI] DNA barcode). A total of 1,576 specimens of 10 genera and 35 species, mostly adult stages, were collected. A total of 225 COI DNA barcode sequences were analyzed; most species formed well-supported groups in the neighbor joining, maximum likelihood, and Bayesian inference trees. The intraspecific Kimura 2-parameter (K2P) genetic distance averaged 1.52%. An intraspecific K2P distance of 6.20% was observed in Anopheles crucians s.l., while a deep split was identified in Culex erraticus and Cx. conspirator. This study showed that COI DNA barcodes offer a reliable approach to support mosquito species identification in Mexico.

Idioma: Inglés

DOI: 10.2987/21-6967

Año: 2021

Publicado en: JOURNAL OF THE AMERICAN MOSQUITO CONTROL ASSOCIATION 37, 4 (2021), 198-207
ISSN: 8756-971X

Factor impacto JCR: 1.0 (2021)

Categ. JCR: ENTOMOLOGY rank: 74 / 100 = 0.74 (2021) - Q3 - T3

Factor impacto CITESCORE: 1.5 - Medicine (Q3) - Agricultural and Biological Sciences (Q3)

Factor impacto SCIMAGO: 0.379 - Ecology, Evolution, Behavior and Systematics (Q3) - Public Health, Environmental and Occupational Health (Q3) - Insect Science (Q3)

Tipo y forma: Article (Published version)

Área (Departamento): Área Sanidad Animal (Dpto. Patología Animal)