Determination and Isolation of Immune Populations from Brain Tumor Microenvironments

Godino, Javier ; Schuhmacher, Alberto J. (Universidad de Zaragoza)

Resumen: Flow cytometry analysis and fluorescence-activated cell sorting (FACS) allow the determination and isolation of different cell types from a given tumor sample. Here we describe and comment a method consisting of the preparation of a single cell suspension from a freshly dissected mouse brain tumor mass, staining with a combination of fluorescently labeled antibodies and analysis by flow cytometry to determine, characterize, and isolate different immune populations.

Idioma: Inglés

DOI: 10.1007/978-1-4939-8885-3_12

Año: 2018

Publicado en: Methods in Molecular Biology 1884 (2018), 177-188
ISSN: 1064-3745

Factor impacto SCIMAGO: 0.605 - Molecular Biology (Q3) - Genetics (Q3)

Tipo y forma: Article (PostPrint)

Área (Departamento): Área Bioquímica y Biolog.Mole. (Dpto. Bioq.Biolog.Mol. Celular)