000170379 001__ 170379
000170379 005__ 20260420103354.0
000170379 0247_ $$2doi$$a10.2174/0118740707389100251014053002
000170379 0248_ $$2sideral$$a148919
000170379 037__ $$aART-2025-148919
000170379 041__ $$aeng
000170379 100__ $$aDoghbri, Lamia
000170379 245__ $$aCam β-NGf supplementation: a promising approach to preserve dromedary camel sperm quality during short-term storage
000170379 260__ $$c2025
000170379 5060_ $$aAccess copy available to the general public$$fUnrestricted
000170379 5203_ $$aIntroduction
The role of camel βeta-Nerve Growth Factor protein (Cam β-NGF), isolated from camel seminal plasma, in preserving sperm quality remains poorly defined. This study aimed to evaluate the impact of Cam β-NGF on mass motility, viability, and membrane integrity in dromedary (Camelus dromedarius) semen stored for 24 hours at 4 °C.
Methods
The semen samples were treated with varying concentrations of Cam β-NGF (10, 100, 500, and 1000 ng/mL) after preservation. Ten semen samples with mass motility ≥ 3 and viability ≥ 60% were selected, washed, suspended in HBSS, and treated with TCF-EY. After 24 hours of storage at 4 °C, samples were maintained at 36 °C with Cam β-NGF supplementation for 60 minutes. Mass motility, viability, and membrane integrity were assessed at T0, T30, and T60 minutes.
Results
The results revealed a significant decline in sperm quality traits after 24 hours of storage at 4 °C. However, mass motility was conserved after 30 minutes of incubation with 1000 ng/mL and 500 ng/mL of Cam β-NGF (p < 0.05). On the other hand, viability was significantly higher after 60 minutes of incubation with 1000, 500, and 100 ng/mL of Cam β-NGF compared to the control extender and 10 ng/mL supplementation (p < 0.05). Additionally, membrane integrity was significantly maintained at T30 and T60 minutes with 1000, 500, and 100 ng/mL of Cam β-NGF (p < 0.05).
Discussion
Short‐term storage at 4 °C markedly reduced dromedary camel sperm motility, viability, and membrane integrity, consistent with cold‐induced oxidative stress. Adding purified Cam β-NGF dose-dependently preserved these parameters over 1 h of incubation, indicating its value as a protective supplement for semen storage.
Conclusion
Cam β-NGF helps protect and maintain sperm traits during short-term storage; it seems to reduce alterations that might lower sperm quality. Thus, supplementing semen with Cam β-NGF may help maintain sperm quality, offering potential benefits for artificial insemination programs in dromedaries.
000170379 536__ $$9info:eu-repo/grantAgreement/ES/DGA/A07-17R
000170379 540__ $$9info:eu-repo/semantics/openAccess$$aby$$uhttps://creativecommons.org/licenses/by/4.0/deed.es
000170379 655_4 $$ainfo:eu-repo/semantics/article$$vinfo:eu-repo/semantics/publishedVersion
000170379 700__ $$aAtigui, Moufida
000170379 700__ $$aSalhi, Imed
000170379 700__ $$0(orcid)0000-0003-3929-5064$$aCarvajal-Serna, Melissa
000170379 700__ $$0(orcid)0000-0003-1997-4262$$aCasao, Adriana$$uUniversidad de Zaragoza
000170379 700__ $$aDbara, Mohamed
000170379 700__ $$0(orcid)0000-0002-2312-6402$$aPérez Pe, Rosaura María$$uUniversidad de Zaragoza
000170379 700__ $$aHammadi, Mohamed
000170379 700__ $$aKdidi, Samia
000170379 7102_ $$11002$$2819$$aUniversidad de Zaragoza$$bDpto. Bioq.Biolog.Mol. Celular$$cÁrea Zoología
000170379 773__ $$g19 (2025), e18740707389100 [9 p.]$$tOpen Biotechnology Journal$$x1874-0707
000170379 8564_ $$s1552993$$uhttps://zaguan.unizar.es/record/170379/files/texto_completo.pdf$$yVersión publicada
000170379 8564_ $$s2300594$$uhttps://zaguan.unizar.es/record/170379/files/texto_completo.jpg?subformat=icon$$xicon$$yVersión publicada
000170379 909CO $$ooai:zaguan.unizar.es:170379$$particulos$$pdriver
000170379 951__ $$a2026-04-18-10:48:23
000170379 980__ $$aARTICLE